HLA-A2 antibody [BB7.2] (Low endotoxin, azide free)
|Applications||Blocking, FACS, IHC-Fr, IP, Functional Assay|
| Blocking||Assay-dependent dilution
| IHC (Frozen sections)||Assay-dependent dilution
| Immunoprecipitation||Assay-dependent dilution
Flow cytometry/FACS : Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood.Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
(Please refer to the vial label for the specific concentration)|
|Purification||Protein A purified|
|Purification Note||From tissue culture supernatant|
|Full Name||major histocompatibility complex, class I, A|
|Product Description||Mouse monoclonal antibody [BB7.2] to HLA-A2|
|Background||HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq, Jul 2008]|
|Synonyms||HLA-A Antibody , HLAA Antibody|
|Specificity||The epitope recognized by this antibody has been studied extensively and would appear to include the carboxy-terminus of the alpha-2 helix and a turn on one of the underlying beta strands. Clone BB7.2 is reported to inhibit MHC restricted cellular cytotoxicity..|
|Immunogen||Papain solubilised HLA-A2|