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HMGA1 antibody, C-term

Anti-HMGA1 antibody, C-term used in Western Blot (WB). GTX80558
Anti-HMGA1 antibody, C-term used in Flow cytometry (FACS). GTX80558
Anti-HMGA1 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80558
Anti-HMGA1 antibody, C-term used in Western Blot (WB). GTX80558

Cat. No. GTX80558

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS

Reactivity

 Human
Package
400 μl ($399)
See all HMGA1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:10-1:50
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Hamster(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 64-93 amino acids from the C-terminal region of human HMGA1.

Purification

Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

high mobility group AT-hook 1 , HMG-R , HMGA1A , HMGIY

Cellular Localization

Nucleus

Background

This gene encodes a chromatin-associated protein involved in the regulation of gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of AT-rich regions in double-stranded DNA. Multiple transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene have been identified on multiple chromosomes. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-HMGA1 antibody, C-term used in Western Blot (WB). GTX80558

GTX80558 WB Image

WB analysis of K562 cell lysate (35ug/lane) using GTX80558 HMGA1 antibody, C-term.

Anti-HMGA1 antibody, C-term used in Flow cytometry (FACS). GTX80558

GTX80558 FACS Image

FACS analysis of MCF-7 cells using GTX80558 HMGA1 antibody, C-term.
Top histogram : negative control
Bottom histogram : MCF-7 cells

Anti-HMGA1 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80558

GTX80558 ICC/IF Image

ICC/IF analysis of A549 cells using GTX80558 HMGA1 antibody, C-term.
Green : HMGA1
Red : Actin filament
Fixation : 4% paraformaldehyde (PFA) (20 min)
Permeabilization : Triton X-100 (0.1%, 10 min)
Dilution : 1:25

Anti-HMGA1 antibody, C-term used in Western Blot (WB). GTX80558

GTX80558 WB Image

WB analysis of NCCIT whole cell lysate using GTX80558 HMGA1 antibody, C-term.
Loading : 20 μg per lane
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399