|Applications||ICC/IF, IHC, IHC-P, WB|
Not tested in other applications.
| Immunohistochemistry||Assay-dependent dilution
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||25 kDa (note)
|Positive Controls||293T , A431 , HeLa , HepG2 , A375 , NIH-3T3 , JC , BCL-1 , C2C12 , Raw264.7 , PC-12|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||high-mobility group box 1|
|Product Description||Rabbit Polyclonal antibody to HMGB1 (high-mobility group box 1)|
|Background||DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. |
|Synonyms||DKFZp686A04236 antibody, HMG1 antibody, HMG3 antibody, SBP-1 antibody, HMGB1 antibody, HMG-1 antibody, Amphoterin antibody, high-mobility group box 1 antibody, high mobility group protein B1 antibody, high mobility group protein 1 antibody, Sulfoglucuronyl carbohydrate binding protein antibody, high-mobility group (nonhistone chromosomal) protein 1 antibody|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human HMGB1. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Pig, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Xenopus laevis, Dog, Rabbit, Chicken, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Xenopus laevis (93%), Dog (100%), Rabbit (100%), Chicken (94%), Rhesus Monkey (100%), Bovine (100%)|