|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||24 kDa (note)
|Positive Controls||293T , A431 , HeLa , HepG2 , A375 , mouse brain , rat brain|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||high-mobility group box 2|
|Product Description||Rabbit Polyclonal antibody to HMGB1/HMGB2 (high-mobility group box 2)|
|Background||This gene encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination. [provided by RefSeq]|
|Synonyms||HMG2 antibody, HMGB2 antibody, high mobility group protein 2 antibody, high-mobility group (nonhistone chromosomal) protein 2 antibody, high mobility group protein B2 antibody, HMG-2 antibody, high-mobility group box 2 antibody|
|Immunogen||Full length human HMGB1/HMGB2 Recombinant protein.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Pig, Chicken, Bovine|
|Predict Reactivity Note||Pig (99%), Chicken (82%), Bovine (100%)|