|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||25 kDa (note)
|Positive Controls||293T , A431 , HeLa , HepG2 , Molt-4 , Raji , mouse liver|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||hypoxanthine phosphoribosyltransferase 1|
|Product Description||Rabbit Polyclonal antibody to HPRT (hypoxanthine phosphoribosyltransferase 1)|
|Background||HPRT1 has a central role in the generation of purine nucleotides through the purine salvage pathway. HPRT1 catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate (Keebaugh et al., 2007 [PubMed 16928426]).[supplied by OMIM]|
|Synonyms||HGPRT antibody, HPRT antibody, HPRT1 antibody, hypoxanthine-guanine phosphoribosyltransferase antibody, HGPRTase antibody, hypoxanthine phosphoribosyltransferase 1 antibody|
|Cellular Localization||Cytoplasm |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human HPRT. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Zebrafish, Xenopus laevis, Dog, Pig, Rabbit, Chicken, Chimpanzee, Bovine|
|Predict Reactivity Note||Zebrafish (93%), Xenopus laevis (92%), Dog (96%), Pig (97%), Rabbit (98%), Chicken (94%), Chimpanzee (99%), Bovine (94%)|