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HSD11B1 antibody

Anti-HSD11B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104626
Anti-HSD11B1 antibody used in Western Blot (WB). GTX104626
Anti-HSD11B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX104626

Cat. No. GTX104626

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Sheep
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

HepG2

PROPERTIES

Form

Liquid

Buffer

PBS, 40% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human HSD11B1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240388

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hydroxysteroid 11-beta dehydrogenase 1 , 11-DH , 11-beta-HSD1 , CORTRD2 , HDL , HSD11 , HSD11B , HSD11L , SDR26C1

Cellular Localization

Endoplasmic reticulum membrane

Background

The protein encoded by this gene is a microsomal enzyme that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. In addition, the encoded protein can catalyze the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol can lead to central obesity, and a particular variation in this gene has been associated with obesity and insulin resistance in children. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HSD11B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104626

GTX104626 ICC/IF Image

HSD11B1 antibody detects HSD11B1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HSD11B1 protein stained by HSD11B1 antibody (GTX104626) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

Anti-HSD11B1 antibody used in Western Blot (WB). GTX104626

GTX104626 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
12% SDS PAGE
GTX104626 diluted at 1:1000

Anti-HSD11B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX104626

GTX104626 IHC-P Image

HSD11B1 antibody detects HSD11B1 protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
HSD11B1 antibody (GTX104626) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169