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HSP27 antibody

KO/KD
Anti-HSP27 antibody used in Western Blot (WB). GTX101145
Anti-HSP27 antibody used in Western Blot (WB). GTX101145
Anti-HSP27 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101145
Anti-HSP27 antibody used in Immunoprecipitation (IP). GTX101145
Anti-HSP27 antibody used in Western Blot (WB). GTX101145
Anti-HSP27 antibody used in Western Blot (WB). GTX101145
Anti-HSP27 antibody used in IHC (Paraffin sections) (IHC-P). GTX101145

Cat. No. GTX101145

Reference 7
4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all HSP27 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS Assay dependent
IP 1:100-1:1000
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

Mouse liver , 293T , A431 , HeLa , HepG2 , HT-29

Predict Reactivity

Bovine, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HSP27. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240967

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein family B (small) member 1 , CMT2F , HEL-S-102 , HMN2B , HS.76067 , HSP27 , HSP28 , Hsp25 , SRP27

Cellular Localization

Cytoplasm , Nucleus , Spindle

Background

The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HSP27 antibody used in Western Blot (WB). GTX101145

GTX101145 WB Image

KO/KD

Non-transfected (–) and transfected (+) HT-29 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 antibody (GTX101145) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HSP27 antibody used in Western Blot (WB). GTX101145

GTX101145 WB Image

KO/KD

Wild-type (WT) and HSP27 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 antibody (GTX101145) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HSP27 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101145

GTX101145 ICC/IF Image

HSP27 antibody detects HSP27 protein at cytoplasm and cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HSP27 protein stained by HSP27 antibody (GTX101145) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.
Anti-HSP27 antibody used in Immunoprecipitation (IP). GTX101145

GTX101145 IP Image

Immunoprecipitation of HSP27 protein from HeLa whole cell extracts using 5 μg of HSP27 antibody (GTX101145).
Western blot analysis was performed using HSP27 antibody (GTX101145).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-HSP27 antibody used in Western Blot (WB). GTX101145

GTX101145 WB Image

Sample (50 μg of whole cell lysate)
A: mouse liver
12% SDS PAGE
GTX101145 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HSP27 antibody used in Western Blot (WB). GTX101145

GTX101145 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 antibody (GTX101145) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HSP27 antibody used in IHC (Paraffin sections) (IHC-P). GTX101145

GTX101145 IHC-P Image

HSP27 antibody detects HSP27 protein at cytoplasm in human lung papillary carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung papillary carcinoma.
HSP27 antibody (GTX101145) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Cui X et al. Dis Model Mech 2022; (Epub) Exercise suppresses mouse systemic AApoAII amyloidosis through enhancement of the p38 MAPK signaling pathway.
Application : WB
Reactivity : Mouse
Cheung CHY et al. J Biomed Sci 2020; 27 (1) : 75 ZNF322A-mediated protein phosphorylation induces autophagosome formation through modulation of IRS1-AKT glucose uptake and HSP-elicited UPR in lung cancer.
Xu YM et al. Toxicol Res (Camb) 2019; 8 (6) : 994-1001 Cadmium telluride quantum dot-exposed human bronchial epithelial cells: a further study of the cellular response by proteomics.
Xu YM et al. Oncotarget 2016; 7 (5) : 6146-58 Proteome profiling of cadmium-induced apoptosis by antibody array analyses in human bronchial epithelial cells.
Reactivity : Human
Huang HJ et al. Mol Biosyst 2014; (Epub) Proteomic analysis of rhein-induced cyt: ER stress mediates cell death in breast cancer cells.
Application : WB
Reactivity : Human
Show More +
Submit a Reference

REVIEW

HSP27 antibody Cat. No. GTX101145

Rating
( Average 4.5 based on 4 users reviews)
Application
Western Blot(WB)
( Average 4.5 based on 2 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 4 based on 1 users reviews)
Flow cytometry(FACS)
( Average 5 based on 1 users reviews)
Anti-HSP27 antibody used in Western Blot (WB). GTX101145
Date : Anonymous submitted on 06-Apr-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : HT29 cells
Amount used : 50 μg
Blocking : 5% skim milk in PBST, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Lane Description : Lane 1: shLacZ (virus infection to silence LacZ gene), Lane 2: shHSP27 (virus infection to silence HSP27 gene)
Anti-HSP27 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101145
Date : Anonymous submitted on 25-Sep-2015
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : Mesenchymal stem cells
Blocking : 3% BSA, 25°C, 2Hr
Fixation : 4% paraformadehyde
Permeabilization /Antigen retrieval : 0.5% Triton X-100
Primary Antibody : 1:100, 4°C, 16Hr
Anti-HSP27 antibody used in Western Blot (WB). GTX101145
Date : Anonymous submitted on 25-Sep-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : 3A6 cells
Amount used : 54μg
Blocking : 3% BSA, 25°C, 2Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-HSP27 antibody used in Flow cytometry (FACS). GTX101145
Date : Anonymous submitted on 24-Oct-2012
Score :
Application Tested : FACS
Sample Species : Hu
Sample : HT29 cells
Fixation : 0.5% skim milk
Primary Antibody : 1:60, 37°C, 1Hr
Figure Note : Blue : Cell alone + goat anti-rabbit-FITC, Red : Hsp27 antibody + goat anti-rabbit-FITC, Green : Unrelated antibody
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169