|Applications||FACS, ICC/IF, IHC-P, IP, WB|
Not tested in other applications.
| Flow cytometry/FACS||Assay-dependent dilution
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||23 kDa (note)
|Positive Controls||293T , A431 , HeLa , HepG2 , HT-29 , Mouse liver|
(Please refer to the vial label for the specific concentration)|
|Purification||Affinity purified by Protein A.|
|Full Name||heat shock 27kDa protein 1|
|Product Description||Rabbit Polyclonal antibody to HSP27 (heat shock 27kDa protein 1)|
|Background||The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq]|
|Synonyms||CMT2F antibody, DKFZp586P1322 antibody, HMN2B antibody, HS.76067 antibody, HSP27 antibody, HSP28 antibody, Hsp25 antibody, SRP27 antibody, HSPB1 antibody, 28 kDa heat shock protein antibody, stress-responsive protein 27 antibody, heat shock protein beta-1 antibody, heat shock 27kD protein 1 antibody, HSP 27 antibody, heat shock 27 kDa protein antibody, estrogen-regulated 24 kDa protein antibody, heat shock 27kDa protein 1 antibody|
|Cellular Localization||Cytoplasm , Nucleus , Spindle |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human HSP27. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Dog, Pig, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Dog (86%), Pig (86%), Rhesus Monkey (100%), Bovine (83%)|