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  • HSP47 antibody [N2C2], Internal

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1 .GTX103011 HSP47 antibody [N2C2], Internal

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HSP47 antibody [N2C2], Internal


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsIHC-P, WB
  • Species reactivityHuman, Mouse
Cat No: GTX103011
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
Add to cart
 
  • Anti-HSP47 antibody [N2C2], Internal used in IHC (Formalin-fixed paraffin-embedded sections). GTX103011
  • Anti-HSP47 antibody [N2C2], Internal used in Western Blot (WB). GTX103011
  • Anti-HSP47 antibody [N2C2], Internal used in IHC (Formalin-fixed paraffin-embedded sections). GTX103011
  • Anti-HSP47 antibody [N2C2], Internal used in Western Blot (WB). GTX103011
  • Anti-HSP47 antibody [N2C2], Internal used in Western Blot (WB). GTX103011
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  • Application Information: HSP47 antibody [N2C2], Internal

Catalog Number GTX103011
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Product Name HSP47 antibody [N2C2], Internal
See all HSP47 products
ApplicationsIHC-P, WB
Application Note
Suggested dilutionReference
IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*  
Western Blot (WB)1:500-1:3000*  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size46 kDa (note) IsoForm (2)
Positive ControlsNIH-3T3 , A431 , HeLa , HepG2 , U87-MG , SK-N-SH , IMR32 , SK-N-AS
Form SuppliedLiquid
Concentration0.37 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: HSP47 antibody [N2C2], Internal

Full Nameserpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
BackgroundThis gene encodes a member of the serpin superfamily of serine proteinase inhibitors. Its expression is induced by heat shock. The protein localizes to the endoplasmic reticulum lumen and binds collagen; thus it is thought to be a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to this protein have been found in patients with rheumatoid arthritis. [provided by RefSeq]
SynonymsAsTP3 antibody, CBP1 antibody, CBP2 antibody, HSP47 antibody, PPROM antibody, RA-A47 antibody, SERPINH2 antibody, gp46 antibody, SERPINH1 antibody, rheumatoid arthritis antigen A-47 antibody, colligin-1 antibody, rheumatoid arthritis-related antigen RA-A47 antibody, cell proliferation-inducing gene 14 protein antibody, serpin H1 antibody, "serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 2, (collagen-binding protein 2) antibody", "serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) antibody", arsenic-transactivated protein 3 antibody, 47 kDa heat shock protein antibody, colligin-2 antibody, "serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) antibody"
Cellular LocalizationEndoplasmic reticulum lumen
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the center region of human HSP47. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman, Mouse
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
 		Rat, Dog, Pig, Chicken, Sheep, Rhesus Monkey, Chimpanzee, Bovine
Predict Reactivity NoteRat (97%), Dog (98%), Pig (96%), Chicken (88%), Sheep (96%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (96%)
ConjugationUnconjugated

  • Storage Conditions: HSP47 antibody [N2C2], Internal

Storage Buffer0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionStore as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20° C or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
HSP47 antibody [N2C2], Internal validated data
  • Anti-HSP47 antibody [N2C2], Internal used in IHC (Formalin-fixed paraffin-embedded sections). GTX103011
    • GTX103011 IHC-P Image
      HSP47 antibody [N2C2], Internal detects HSP47 protein at cytosol on human breast carcinoma by immunohistochemical analysis.
      Sample: Paraffin-embedded human breast carcinoma.
      HSP47 antibody [N2C2], Internal (GTX103011) dilution: 1:500.

      Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min
      Top
  • Anti-HSP47 antibody [N2C2], Internal used in Western Blot (WB). GTX103011
    • GTX103011 WB Image
      Sample (30 µg of whole cell lysate)
      A: NIH-3T3
      10% SDS PAGE
      GTX103011 diluted at 1:500
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-HSP47 antibody [N2C2], Internal used in IHC (Formalin-fixed paraffin-embedded sections). GTX103011
    • GTX103011 IHC-P Image
      HSP47 antibody [N2C2], Internal detects HSP47 protein at cytosol on mouse kidney by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse kidney.
      HSP47 antibody [N2C2], Internal (GTX103011) dilution: 1:500.

      Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min
      Top
  • Anti-HSP47 antibody [N2C2], Internal used in Western Blot (WB). GTX103011
    • GTX103011 WB Image
      HSP47 antibody [N2C2], Internal detects HSP47 protein by western blot analysis.
      A. 30 µg A431 whole cell lysate/extract
      B. 30 µg HeLa whole cell lysate/extract
      C. 30 µg HepG2 whole cell lysate/extract
      10% SDS-PAGE
      HSP47 antibody [N2C2], Internal (GTX103011) dilution: 1:500
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-HSP47 antibody [N2C2], Internal used in Western Blot (WB). GTX103011
    • GTX103011 WB Image
      HSP47 antibody [N2C2], Internal detects HSP47 protein by western blot analysis.
      A. 30 µg U87-MG whole cell lysate/extract
      B. 30 µg SK-N-SH whole cell lysate/extract
      C. 30 µg IMR32 whole cell lysate/extract
      D. 30 µg SK-N-AS whole cell lysate/extract
      10% SDS-PAGE
      HSP47 antibody [N2C2], Internal (GTX103011) dilution: 1:500
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-HSP47 antibody [N2C2], Internal used in IHC (Formalin-fixed paraffin-embedded sections). GTX103011
    • GTX103011 IHC-P Image
      HSP47 antibody [N2C2], Internal detects HSP47 protein at cytosol on human breast carcinoma by immunohistochemical analysis.
      Sample: Paraffin-embedded human breast carcinoma.
      HSP47 antibody [N2C2], Internal (GTX103011) dilution: 1:500.

      Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min
      Top
  • Anti-HSP47 antibody [N2C2], Internal used in Western Blot (WB). GTX103011
    • GTX103011 WB Image
      Sample (30 µg of whole cell lysate)
      A: NIH-3T3
      10% SDS PAGE
      GTX103011 diluted at 1:500
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-HSP47 antibody [N2C2], Internal used in IHC (Formalin-fixed paraffin-embedded sections). GTX103011
    • GTX103011 IHC-P Image
      HSP47 antibody [N2C2], Internal detects HSP47 protein at cytosol on mouse kidney by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse kidney.
      HSP47 antibody [N2C2], Internal (GTX103011) dilution: 1:500.

      Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min
      Top
  • Anti-HSP47 antibody [N2C2], Internal used in Western Blot (WB). GTX103011
    • GTX103011 WB Image
      HSP47 antibody [N2C2], Internal detects HSP47 protein by western blot analysis.
      A. 30 µg A431 whole cell lysate/extract
      B. 30 µg HeLa whole cell lysate/extract
      C. 30 µg HepG2 whole cell lysate/extract
      10% SDS-PAGE
      HSP47 antibody [N2C2], Internal (GTX103011) dilution: 1:500
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-HSP47 antibody [N2C2], Internal used in Western Blot (WB). GTX103011
    • GTX103011 WB Image
      HSP47 antibody [N2C2], Internal detects HSP47 protein by western blot analysis.
      A. 30 µg U87-MG whole cell lysate/extract
      B. 30 µg SK-N-SH whole cell lysate/extract
      C. 30 µg IMR32 whole cell lysate/extract
      D. 30 µg SK-N-AS whole cell lysate/extract
      10% SDS-PAGE
      HSP47 antibody [N2C2], Internal (GTX103011) dilution: 1:500
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
Back to Top
Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensSERPINH1871P50454, B3KVJ3600943, 610504NP_00119394346.45
Homo sapiensSERPINH1871P50454, B3KVJ3600943, 610504NM_001235NP_00122646.45

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Mus musculusSerpinh112406Q5U4D0NM_009825NP_03395546.54
Mus musculusSerpinh112406Q5U4D0NP_00127270546.54
Mus musculusSerpinh112406Q5U4D0NM_001111043NP_00110451346.54
Mus musculusSerpinh112406Q5U4D0NM_001111044NP_00110451446.54
Rattus norvegicusSerpinh129345P29457NM_017173NP_05886946.52
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-Thimerosal.pdf

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