|Applications||ELISA, ICC/IF, IHC-P, WB|
Not tested in other applications.
| ELISA||Assay-dependent dilution
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||61 kDa (note)
|Positive Controls||293T , A431 , HeLa , HepG2 , mouse brain , rat brain|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||heat shock 60kDa protein 1 (chaperonin)|
|Product Description||Rabbit Polyclonal antibody to HSP60 (heat shock 60kDa protein 1 (chaperonin))|
|Background||This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Two pseudogenes, both located on chromosome 8, have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq]|
|Synonyms||CPN60 antibody, GROEL antibody, HLD4 antibody, HSP-60 antibody, HSP60 antibody, HSP65 antibody, HuCHA60 antibody, SPG13 antibody, HSPD1 antibody, 60 kDa chaperonin antibody, mitochondrial matrix protein P1 antibody, chaperonin 60 antibody, short heat shock protein 60 Hsp60s1 antibody, P60 lymphocyte protein antibody, heat shock protein 65 antibody, 60 kDa heat shock protein, mitochondrial antibody, heat shock 60kDa protein 1 (chaperonin) antibody|
|Cellular Localization||Mitochondrion matrix |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human HSP60. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat, Hamster|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Zebrafish, Xenopus laevis, Pig, Chicken, Rhesus Monkey, Chimpanzee, Bovine|
|Predict Reactivity Note||Zebrafish (85%), Xenopus laevis (91%), Pig (99%), Chicken (94%), Rhesus Monkey (97%), Chimpanzee (99%), Bovine (98%)|