GeneTex

  • Country / Location Selection

United States (US)

Hexokinase II antibody

Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX111525
Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX111525
Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX111525
Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX111525
Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX111525
Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX111525
Anti-Hexokinase II antibody used in Western Blot (WB). GTX111525
Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX111525
Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX111525
Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX111525
Anti-Hexokinase II antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111525
Anti-Hexokinase II antibody used in Western Blot (WB). GTX111525
Anti-Hexokinase II antibody used in Western Blot (WB). GTX111525

Cat. No. GTX111525

Reference 9
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Hexokinase II products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

Predict Reactivity

Zebrafish, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.62 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Hexokinase II. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10724113

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hexokinase 2 , HKII , HXK2

Cellular Localization

Mitochondrion outer membrane

Background

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX111525

GTX111525 IHC-P Image

Hexokinase II antibody detects Hexokinase II protein at cytoplasm in rat stomach by immunohistochemical analysis.
Sample: Paraffin-embedded rat stomach.
Hexokinase II antibody (GTX111525) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX111525

GTX111525 IHC-P Image

Hexokinase II antibody detects Hexokinase II protein at cytosol on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
Hexokinase II antibody (GTX111525) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX111525

GTX111525 IHC-P Image

Hexokinase II antibody detects Hexokinase II protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
Hexokinase II stained by Hexokinase II antibody (GTX111525) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX111525

GTX111525 IHC-P Image

Hexokinase II antibody detects Hexokinase II protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
Hexokinase II stained by Hexokinase II antibody (GTX111525) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX111525

GTX111525 IHC-P Image

Hexokinase II antibody detects Hexokinase II protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat muscle.
Hexokinase II stained by Hexokinase II antibody (GTX111525) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX111525

GTX111525 IHC-P Image

Hexokinase II antibody detects Hexokinase II protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Hexokinase II stained by Hexokinase II antibody (GTX111525) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Hexokinase II antibody used in Western Blot (WB). GTX111525

GTX111525 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hk2 antibody (GTX111525) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX111525

GTX111525 IHC-P Image

Hexokinase II antibody detects HK2 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
Hexokinase II antibody (GTX111525) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX111525

GTX111525 IHC-P Image

Hexokinase II antibody detects Hexokinase II protein at cytoplasm in rat heart by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
Hexokinase II antibody (GTX111525) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX111525

GTX111525 IHC-P Image

Hexokinase II antibody detects Hexokinase II protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
Hexokinase II stained by Hexokinase II antibody (GTX111525) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Hexokinase II antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111525

GTX111525 ICC/IF Image

Hexokinase II antibody detects Hexokinase II protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Hexokinase II stained by Hexokinase II antibody (GTX111525) diluted at 1:2000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-Hexokinase II antibody used in Western Blot (WB). GTX111525

GTX111525 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Hk2 antibody (GTX111525) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Hexokinase II antibody used in Western Blot (WB). GTX111525

GTX111525 WB Image

The data was published in the 2022 in Int J Mol Sci. PMID: 35328751

REFERENCE

Application Reference

Izabela Bary?a et al. Int J Mol Sci 2022; 23 (6) The WWOX/HIF1A Axis Downregulation Alters Glucose Metabolism and Predispose to Metabolic Disorders.
Application : WB
Reactivity : Human
Huang CY et al. Life (Basel) 2021; 11 (9) Calcitriol Suppresses Warburg Effect and Cell Growth in Human Colorectal Cancer Cells.
Application : WB
Reactivity : Human
Harada Y et al. Cell Rep 2020; 33 (2) : 108261 Glycometabolic Regulation of the Biogenesis of Small Extracellular Vesicles.
Application : WB
Reactivity : Mouse
Lee YR et al. Kaohsiung J Med Sci 2020; 36 (11) : 911-919 Regulation of autophagy, glucose uptake, and glycolysis under dengue virus infection.
Application : WB
Reactivity : Human
Takino JI et al. Sci Rep 2019; 9 (1) : 16717 The inhibition of Bax activation-induced apoptosis by RasGRP2 via R-Ras-PI3K-Akt signaling pathway in the endothelial cells.
Application : WB
Reactivity : Human
Show More +
Submit a Reference

REVIEW

Hexokinase II antibody Cat. No. GTX111525

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-Hexokinase II antibody used in Western Blot (WB). GTX111525
Date : Anonymous submitted on 23-Oct-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : Placenta tissue lysate
Amount used : 60 μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 18Hr
Submit a Review
Application Reference
Izabela Bary?a et al. Int J Mol Sci 2022; 23 (6) The WWOX/HIF1A Axis Downregulation Alters Glucose Metabolism and Predispose to Metabolic Disorders.
Application : WB
Reactivity : Human
Huang CY et al. Life (Basel) 2021; 11 (9) Calcitriol Suppresses Warburg Effect and Cell Growth in Human Colorectal Cancer Cells.
Application : WB
Reactivity : Human
Harada Y et al. Cell Rep 2020; 33 (2):108261 Glycometabolic Regulation of the Biogenesis of Small Extracellular Vesicles.
Application : WB
Reactivity : Mouse
Lee YR et al. Kaohsiung J Med Sci 2020; 36 (11):911-919 Regulation of autophagy, glucose uptake, and glycolysis under dengue virus infection.
Application : WB
Reactivity : Human
Takino JI et al. Sci Rep 2019; 9 (1):16717 The inhibition of Bax activation-induced apoptosis by RasGRP2 via R-Ras-PI3K-Akt signaling pathway in the endothelial cells.
Application : WB
Reactivity : Human
Lee CH et al. Mol Imaging Biol 2018; (Epub) Adenine Nucleotide Translocase 2 as an Enzyme Related to [18F] FDG Accumulation in Various Cancers.
Application : WB
Reactivity : Human
Yang T et al. Oncogene 2018; (Epub) pim2-mediated phosphorylation of hexokinase 2 is critical for tumor growth and * resistance in breast cancer.
Application : WB
Reactivity : Human
Huang YP et al. Clin Exp Pharmacol Physiol 2017; (Epub) Proteomic analysis of oral cancer reveals new potential therapeutic targets involved in the Warburg effect.
Application : WB
Reactivity : Human
Jan CI et al. Int J Biol Sci? 2016; 12 (7):786-798 Fenofibrate Suppresses Oral Tumorigenesis via Reprogramming Metabolic Processes: Potential Drug Repurposing for Oral Cancer.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169