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Hif1ab antibody

Orthogonal
Anti-Hif1ab antibody used in Western Blot (WB). GTX131826

Cat. No. GTX131826

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all zebrafish Hif1ab products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Positive Control

zebrafish embryos (untreated) , zebrafish embryos (treated UV irradiation)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.68 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of zebrafish Hif1ab. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886524

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hypoxia inducible factor 1 subunit alpha b , hif1a , wu:fb63a10 , zgc:55405

Database

Research Area

DATA IMAGES

Anti-Hif1ab antibody used in Western Blot (WB). GTX131826

GTX131826 WB Image

Orthogonal

Hif1ab antibody detects Hif1ab protein by western blot analysis. Un-treated (-) and treated (+, UVC treatment for ) zebrafish embryos whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hif1ab antibody (GTX131826) at a dilution of 1:1000.

REFERENCE

Application Reference

Claudio Laquatra et al. Cell Death Dis 2021; 12 (5) : 434 HIF1α-dependent induction of the mitochondrial chaperone TRAP1 regulates bioenergetic adaptations to hypoxia.
Application : WB
Reactivity : Zebrafish
Newman M et al. Hum Mol Genet 2020; 29 (14) : 2379-2394 Accelerated loss of hypoxia response in zebrafish with familial Alzheimer's disease-like mutation of presenilin 1.
Application : WB
Reactivity : Zebrafish
Morgan Newman et al. bioRxiv 2019; (Epub) Accelerated loss of hypoxia response and biased allele expression in zebrafish with Alzheimer's disease-like mutations.
Application : WB
Reactivity : Zebrafish
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169