APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:20000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
mouse brain , rat brain , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2 , HCT116 (30uM cisplatin for 24hr)
Predict Reactivity
Bovine, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.41 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Histone H2A.X. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1950472
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
,H2A histone family member X,gamma H2A.X,gamma H2AX,H2A.X,H2A/X,H2AX,H2AFX,
Cellular Localization
Nucleus
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX108272 IHC-P Image
Histone H2A.X antibody detects Histone H2A.X protein at nucleus in human esophagus cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human esophagus cancer.
Histone H2A.X antibody (GTX108272) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX108272 IHC-P Image
Histone H2A.X antibody detects Histone H2A.X protein at nucleus in human cervical cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical cancer.
Histone H2A.X antibody (GTX108272) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX108272 IHC-P Image
Histone H2A.X antibody detects Histone H2A.X protein at nucleus on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
Histone H2A.X antibody (GTX108272) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX108272 IHC-P Image
Histone H2A.X antibody detects Histone H2A.X protein at nucleus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
Histone H2A.X antibody (GTX108272) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX108272 IHC-P Image
Histone H2A.X antibody detects Histone H2A.X protein at nucleus on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
Histone H2A.X antibody (GTX108272) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX108272 ICC/IF Image
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using Histone H2A.X (GTX108272) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
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GTX108272 IHC-P Image
Histone H2A.X antibody detects Histone H2A.X protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.Histone H2A.X stained by Histone H2A.X antibody (GTX108272) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX108272 IP Image
Histone H2A.X antibody immunoprecipitates H2AFX protein in IP experiments.
IP samples: Jurkat whole cell extract
A. 40 μg Jurkat whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of H2AFX protein by 4 μg Histone H2A.X antibody (GTX108272)
15 % SDS-PAGE
The immunoprecipitated H2AFX protein was detected by Histone H2A.X antibody (GTX108272) diluted at 1:2000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
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GTX108272 WB Image
Histone H2A.X antibody detects Histone H2A.X protein by western blot analysis.
A. 50 μg rat brain extract
12% SDS-PAGE
Histone H2A.X antibody (GTX108272) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX108272 ICC/IF Image
Histone H2A.X antibody detects Histone H2A.X protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Histone H2A.X stained by Histone H2A.X antibody (GTX108272) diluted at 1:2000.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar= 10 μm.
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GTX108272 WB Image
Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X antibody (GTX108272) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX108272 WB Image
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X antibody (GTX108272) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX108272 WB Image
Histone H2A.X antibody detects Histone H2A.X protein by western blot analysis.
A. 50 μg mouse brain extract
12% SDS-PAGE
Histone H2A.X antibody (GTX108272) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX108272 WB Image
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X antibody (GTX108272) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX108272 WB Image
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X antibody (GTX108272) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
REVIEW
Histone H2A.X antibody
Cat. No. GTX108272
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Rating
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( Average 4.5 based on 2 users reviews)
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Application
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Western Blot(WB)
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( Average 4.5 based on 2 users reviews)
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Date :
Anonymous submitted on 04-Jul-2023
Application Tested :
WB
Sample Species :
Hu
Sample :
SUM159 cells with/without treatment
Amount used :
15μg
Blocking :
5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
Date :
Anonymous submitted on 13-May-2015
Application Tested :
WB
Sample Species :
Ms
Sample :
4T1 cells
Amount used :
20μg
Blocking :
4% non-fat milk in TBST, 23°C, 1Hr
Primary Antibody :
1:4000, 4°C, 16Hr
