Histone H2A.XS139ph (phospho Ser139) antibody [GT2311]
|Applications||ICC/IF, IHC-P, IP, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||15 kDa (note)
|Positive Controls||HCT116 (30µM cisplatin treatment for 24hr) , NIH-3T3 (30µM cisplatin treatment for 24hr) , PC-12 (30uM cisplatin treatment for 24h) , U2OS treated UV (50 J/m2)|
(Please refer to the vial label for the specific concentration)|
|Purification||Affinity purified by Protein G.|
|Full Name||H2A histone family, member X|
|Product Description||Mouse Monoclonal antibody to Histone H2A.X (phospho S139) (H2A histone family, member X)|
|Background||Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq]|
|Synonyms||gamma H2AX, H2A.XS139ph,Phospho Histone H2A.X (pS139 )|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Rhesus Monkey, Bovine|
|Predict Reactivity Note||Rhesus Monkey (100%), Bovine (100%)|