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Histone H2B antibody

Anti-Histone H2B antibody used in Western Blot (WB). GTX115955
Anti-Histone H2B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115955
Anti-Histone H2B antibody used in IHC (Paraffin sections) (IHC-P). GTX115955
Anti-Histone H2B antibody used in IHC (Paraffin sections) (IHC-P). GTX115955
Anti-Histone H2B antibody used in Western Blot (WB). GTX115955
Anti-Histone H2B antibody used in Western Blot (WB). GTX115955

Cat. No. GTX115955

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all Histone H2B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Positive Control

293T , A431 , HeLa , HepG2 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12

Predict Reactivity

Bovine, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Histone H2B Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10622324

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Histone H2B antibody used in Western Blot (WB). GTX115955

GTX115955 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2B antibody (GTX115955) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H2B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115955

GTX115955 ICC/IF Image

Histone H2B antibody detects Histone H2B protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min.
Green: Histone H2B protein stained by Histone H2B antibody (GTX115955) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-Histone H2B antibody used in IHC (Paraffin sections) (IHC-P). GTX115955

GTX115955 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using Histone H2B(GTX115955) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H2B antibody used in IHC (Paraffin sections) (IHC-P). GTX115955

GTX115955 IHC-P Image

Histone H2B antibody detects Histone H2B protein at nucleus in mouse middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse middle brain.
Histone H2B antibody (GTX115955) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H2B antibody used in Western Blot (WB). GTX115955

GTX115955 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2B antibody (GTX115955) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H2B antibody used in Western Blot (WB). GTX115955

GTX115955 WB Image

The equivalent of 4 larvae was loaded
A: 120hpf larvae
4-12% NuPAGE Bis Tris precast gel
GTX115955 diluted at 1:1000

REFERENCE

Application Reference

Yu ZC et al. Nucleic Acids Res 2015; Requirement for human Mps1/TTK in oxidative DNA damage repair and cell survival through MDM2 phosphorylation.
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169