Histone H3 antibody

Host / ClonalityRabbit Polyclonal
ApplicationsICC/IF, IHC-P, IP, WB, ChIP assay
Species reactivityHuman, Mouse, Drosophila, Monkey, Rat, Rice
★★★★☆ ( 3 ) ( 10 )

Cat No:

GTX122148

Package	Price	Qty
25 μl	USD 119.00
100 μl	USD 319.00

Add to cart

LinkButton

Datasheet
Star Review (3)
Reference (10)
Bio-info
Protocol & MSDS

Application Information: Histone H3 antibody

Catalog Number

GTX122148

Product Name

Histone H3 antibody

See all Histone H3 products

Applications

ICC/IF, IHC-P, IP, WB, ChIP assay

Application Note

	Suggested dilution	Reference
ChIP assay	Assay-dependent dilution
Immunocytochemistry/ Immunofluorescence	1:100-1:1000*	Y
IHC (Formalin-fixed paraffin-embedded sections)	1:100-1:1000*
Immunoprecipitation	1:100-1:500*
Western blot	1:500-1:10000*	Y

Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.

Predicted Target Size

15 kDa (note)

Positive Controls

293T , A431 , HeLa , HepG2 , PC-12 , Rat2 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12

Form Supplied

Liquid

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration)

Purification

Purified by antigen-affinity chromatography.

Specifications: Histone H3 antibody

Full Name	histone cluster 1, H3a
Product Description	Rabbit Polyclonal antibody to Histone H3 (histone cluster 1, H3a)
Background	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq]
Synonyms	H3/A antibody, H3FA antibody, HIST1H3B antibody, HIST1H3C antibody, HIST1H3D antibody, HIST1H3E antibody, HIST1H3F antibody, HIST1H3G antibody, HIST1H3H antibody, HIST1H3I antibody, HIST1H3J antibody, HIST1H3A antibody, histone H3/j antibody, histone H3/i antibody, histone H3/d antibody, histone H3/a antibody, histone H3/h antibody, histone H3/k antibody, histone H3/l antibody, histone H3.1 antibody, "H3 histone family, member A antibody", histone H3/c antibody, "histone 1, H3a antibody", histone H3/f antibody, histone H3/b antibody, "histone cluster 1, H3a antibody"
Cellular Localization	Nucleus
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H3. The exact sequence is proprietary.
Antigen Species	Human
Species Reactivity	Human, Mouse, Drosophila, Monkey, Rat, Rice
Conjugation	Unconjugated

Storage Conditions: Histone H3 antibody

Storage Buffer	1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage Instruction	Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Notes	For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
Epigenetics > Unmodified histone More Hide

Histone H3 antibody validated data

GTX122148 IHC-P Image

Histone H3 antibody detects Histone H3 protein at nucleus in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Green: Histone H3 antibody (GTX122148) diluted at 1:500.

Red: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.

Top

GTX122148 WB Image

Histone H3 antibody detects Histone H3 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody (GTX122148) diluted at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Top

GTX122148 WB Image

Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody (GTX122148) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Top

GTX122148 WB Image

Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody (GTX122148) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Top

GTX122148 IP Image

Histone H3 antibody immunoprecipitates Histone H3 protein in IP experiments. IP Sample: Raji whole cell lysate/extract A : Control with 3 μg of pre-immune rabbit IgG B : Immunoprecipitation of Histone H3 by 3 μg of Histone H3 antibody (GTX122148) 15% SDS-PAGE The immunoprecipitated Histone H3 protein was detected by Histone H3 antibody (GTX122148) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Top

GTX122148 ICC/IF Image

Histone H3 antibody detects Histone H3 protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: Histone H3 protein stained by Histone H3 antibody (GTX122148) diluted at 1:500.
Blue: Hoechst 33342 staining.

Top

GTX122148 ChIP assay Image

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-Histone H3 antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD.

Top

GTX122148 IHC-P Image

Histone H3 antibody detects Histone H3 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Histone H3 antibody (GTX122148) diluted at 1:500.

Top

GTX122148 IHC-P Image

Histone H3 antibody detects Histone H3 protein at nucleus in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Histone H3 antibody (GTX122148) diluted at 1:500.

Top

GTX122148 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using Histone H3(GTX122148) antibody at 1:500 dilution.

Top

GTX122148 IHC-P Image

Histone H3 antibody detects Histone H3 protein at nucleus in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Green: Histone H3 antibody (GTX122148) diluted at 1:500.

Red: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.

Top

GTX122148 WB Image

Histone H3 antibody detects Histone H3 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody (GTX122148) diluted at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Top

GTX122148 WB Image

Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody (GTX122148) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Top

GTX122148 WB Image

Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody (GTX122148) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Top

GTX122148 IP Image

Histone H3 antibody immunoprecipitates Histone H3 protein in IP experiments. IP Sample: Raji whole cell lysate/extract A : Control with 3 μg of pre-immune rabbit IgG B : Immunoprecipitation of Histone H3 by 3 μg of Histone H3 antibody (GTX122148) 15% SDS-PAGE The immunoprecipitated Histone H3 protein was detected by Histone H3 antibody (GTX122148) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Top

GTX122148 ICC/IF Image

Histone H3 antibody detects Histone H3 protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: Histone H3 protein stained by Histone H3 antibody (GTX122148) diluted at 1:500.
Blue: Hoechst 33342 staining.

Top

GTX122148 ChIP assay Image

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-Histone H3 antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD.

Top

GTX122148 IHC-P Image

Histone H3 antibody detects Histone H3 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Histone H3 antibody (GTX122148) diluted at 1:500.

Top

GTX122148 IHC-P Image

Histone H3 antibody detects Histone H3 protein at nucleus in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Histone H3 antibody (GTX122148) diluted at 1:500.

Top

GTX122148 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using Histone H3(GTX122148) antibody at 1:500 dilution.

Top

StarReview
Histone H3 antibody
Rating : ★★★★☆ ( Average 4.0 based on 3 users reviews)
Western blot(3) :★★★★☆

Anonymous StarReview submitted on 22 Dec 2012
Score : ★★★★★
Application Tested : WB
Sample Species : Monkey
Sample : Vero E6 cells
Amount used(ug/cells) : 100ug
Blocking conditions
Blocking Buffer : 5% milk
Incubation Time & Temperature : 25℃ (℃)/1hr
Primary antibody
Dilution : 1:1000( conc. of dilution )
Incubation Time & Temperature : 4℃ (℃)/12hr

Anonymous StarReview submitted on 22 May 2014
Score : ★★★☆☆
Application Tested : WB
Sample Species : Rice
Sample : rice leaf tissue
Amount used(ug/cells) : 50ug
Blocking conditions
Blocking Buffer : 5% milk in tris buffer
Incubation Time & Temperature : 4 (℃)/1hr
Primary antibody
Dilution : 1:2500( conc. of dilution )
Incubation Time & Temperature : 4 (℃)/overnight

Anonymous StarReview submitted on 10 Mar 2014
Score : ★★★★☆
Application Tested : WB
Sample Species : Human
Sample : primary adipocyte cells
Amount used(ug/cells) : 10ug
Blocking conditions
Blocking Buffer : 5% nonfat milk in TBST(1xTBS, 0.1%Tween 20)
Incubation Time & Temperature : 25 (℃)/3hr
Primary antibody
Dilution : 1:2000( conc. of dilution )
Incubation Time & Temperature : 4 (℃)/overnight

Reference for Histone H3 antibody

Application References	Application	Reactivity
Yang WS et al. SUMO modification of a heterochromatin histone demethylase JMJD2A enables viral gene transactivation and viral replication. PLoS Pathog 13 (2):e1006216 (2017).	WB	Human
Tenreiro P et al. Comparison of simple sucrose and percoll based methodologies for synaptosome enrichment. Anal Biochem 517:1-8 (2016).	WB	Rat
Shih CC et al. Procainamide Inhibits DNA Methylation and Alleviates Multiple Organ Dysfunction in Rats with Endotoxic Shock. PLoS One 11 (9):e0163690 (2016).	WB	Rat
Horke S et al. Novel Paraoxonase 2-Dependent Mechanism Mediating the Biological Effects of the Pseudomonas aeruginosa Quorum-Sensing Molecule N-(3-Oxo-Dodecanoyl)-L-Homoserine Lactone. Infect Immun 83 (9):3369-80 (2015).	WB	Human
Hii HP et al. Distinct Patterns of Wnt3a and Wnt5a Signaling Pathway in the Lung from Rats with Endotoxic Shock. PLoS One 10 (7):e0134492 (2015).	WB	Rat
Li PT et al. Increased Histone Deacetylase Activity Involved in the Suppressed Invasion of Cancer Cells Survived from ALA-Mediated Photodynamic Treatment. Int J Mol Sci 16 (10):23994-4010 (2015).	WB	Human
Li CJ et al. Synergistic protection of N-acetylcysteine and ascorbic acid 2-phosphate on human mesenchymal stem cells against mitoptosis, necroptosis and apoptosis. Sci Rep (Epub ahead of print) (2015).	WB	Human
Huang YC et al. The COP9 Signalosome Converts Temporal Hormone Signaling to Spatial Restriction on Neural Competence. PLoS Genet 10 (11):e1004760 (2014).	ICC/IF	Drosophila
Lee KH et al. A gene expression signature-based approach reveals the mechanisms of action of the Chinese herbal medicine berberine. Sci Rep 4:6394 (2014).	WB	Human
Lee YR et al. Reversine suppresses oral squamous cell carcinoma via cell cycle arrest and concomitantly apoptosis and autophagy. J Biomed Sci 19:9 (2012).	WB	Human

Target Bioinfo/ Predicted Isoform Cross Reactivity
Species	Gene name	Gene ID	SwissProt	OMIM	Isoform (mRNA)	Protein Accession#	MW
Homo sapiens	HIST1H3A	8350	P68431, A5PLR1	602810	NM_003529	NP_003520	15.41