| Catalog Number | GTX128116 |
| Product Name | Histone H3 (phospho Ser10) antibody |
| Full Name | histone cluster 1, H3a |
| Synonyms | H3/A antibody | H3FA antibody | HIST1H3B antibody | HIST1H3C antibody | HIST1H3D antibody | HIST1H3E antibody | HIST1H3F antibody | HIST1H3G antibody | HIST1H3H antibody | HIST1H3I antibody | HIST1H3J antibody | HIST1H3A antibody | histone H3/j antibody | histone H3/i antibody | histone H3/d antibody | histone H3/a antibody | histone H3/h antibody | histone H3/k antibody | histone H3/l antibody | histone H3.1 antibody | H3 histone family, member A antibody | histone H3/c antibody | histone 1, H3a antibody | histone H3/f antibody | histone H3/b antibody | histone cluster 1, H3a antibody |
| Product Description | Rabbit Polyclonal antibody to Histone H3 (phospho Ser10) (histone cluster 1, H3a) |
| Background | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq] |
| Host | Rabbit |
| Clonality | Polyclonal |
| Subclass | IgG |
| Immunogen | Synthetic phospho-peptide corresponding to amino acids residues surrounding the phospho Ser10 of Histone H3 |
| Antigen Species | Human |
| Species Reactivity | Human, Mouse |
| Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Positive Controls | U2OS (100ng/ml Nocodazole treatment for 24hr) | 4T1 (100ng/ml Nocodazole treatment for 24hr) |
| Predicted Target Size(KDa) | 15 (Note) |
| Cellular Localization | Nucleus | |
| Form Supplied | Liquid |
| Concentration | 1 mg/ml |
| Purification | Purified by antigen-affinity chromatography. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
| Storage Instruction | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Notes | For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |