Histone H3S10ph (phospho Ser10) antibody
|Full Name||histone cluster 1, H3a|
|Synonyms||H3/A antibody, H3FA antibody, HIST1H3B antibody, HIST1H3C antibody, HIST1H3D antibody, HIST1H3E antibody, HIST1H3F antibody, HIST1H3G antibody, HIST1H3H antibody, HIST1H3I antibody, HIST1H3J antibody, HIST1H3A antibody, histone H3/j antibody, histone H3/i antibody, histone H3/d antibody, histone H3/a antibody, histone H3/h antibody, histone H3/k antibody, histone H3/l antibody, histone H3.1 antibody, H3 histone family, member A antibody, histone H3/c antibody, histone 1, H3a antibody, histone H3/f antibody, histone H3/b antibody, histone cluster 1, H3a antibody|
|Product Description||Rabbit Polyclonal antibody to Histone H3 (phospho Ser10) (histone cluster 1, H3a)|
|Background||Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq]|
|Immunogen||Synthetic phospho-peptide corresponding to amino acids residues surrounding the phospho Ser10 of Histone H3 |
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Predict Reactivity Note||Chimpanzee (100%)|
|Applications||ICC/IF, IHC-P, IP, WB, ChIP assay|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Positive Controls||U2OS (100ng/ml Nocodazole treatment for 24hr) , 4T1 (100ng/ml Nocodazole treatment for 24hr) , Rat2 whole cell lysate/extract (100 ng/ml Nocodazole treatment for 24 hr)|
|Predicted Target Size||15 kDa (note)|
|Cellular Localization||Nucleus |
|Purification||Purified by antigen-affinity chromatography.|
|Storage Buffer||1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.|
|Storage Instruction||Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.|
|Notes||For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |