Histone H3K27ac (acetyl Lys27) antibody [MABI0309]
|Applications||ELISA, ICC/IF, WB, ChIP assay|
|Application Note||Suggested starting dilutions are as follows: WB : 1:500-1:3000, ICC/IF: 1:100-1:1000, ChIP : 1-5 µg/5 µl Sepharose(Mouse IgG has weak combination with protein A and protein G. Please use the beads which connect anti-mouse IgG antibodies such as anti-mouse IgG sepharose for ChIP). Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
|Positive Controls||HeLa (0.012% DMSO treatment for 18 hr) , HeLa (0.4 µM TSA treatment for 18 hr)|
(Please refer to the vial label for the specific concentration)|
|Purification||Protein A Chromatography|