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Histone H3K9ac (Acetyl Lys9) antibody [GT464]

Anti-Histone H3K9ac (Acetyl Lys9) antibody [GT464] used in Western Blot (WB). GTX630554
Anti-Histone H3K9ac (Acetyl Lys9) antibody [GT464] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630554

Cat. No. GTX630554

Host

Mouse

Clonality

Monoclonal

Clone Name

GT464

Isotype

IgG1

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Positive Control

HeLa (0.012% DMSO treatment for 18 hr) , HeLa (0.4 μM Tricostatin A treatment for 18 hr)

Predict Reactivity

Mouse, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding acetyl Lys9 of human Histone H3. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888219

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

DATA IMAGES

Anti-Histone H3K9ac (Acetyl Lys9) antibody [GT464] used in Western Blot (WB). GTX630554

GTX630554 WB Image

Histone H3K9ac (acetyl Lys9) antibody [GT464] detects Histone H3K9ac (acetyl Lys9) protein by western blot analysis.
A. 30 μg HeLa whole cell lysate/extract (0.012% DMSO treatment for 18 hr)
B. 30 μg HeLa whole cell lysate/extract (0.4 μM Tricostatin A treatment for 18 hr)
15 % SDS-PAGE
Histone H3K9ac (acetyl Lys9) antibody [GT464] (GTX630554) dilution: 1:5000

Anti-Histone H3K9ac (Acetyl Lys9) antibody [GT464] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630554

GTX630554 ICC/IF Image

Histone H3K9ac (acetyl Lys9) antibody [GT464] detects Histone H3K9ac (acetyl Lys9) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H3K9ac (acetyl Lys9) protein stained by Histone H3K9ac (acetyl Lys9) antibody [GT464] (GTX630554) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations