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1 .GTX124486 Hmgb1 antibody

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Hmgb1 antibody


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsIHC-P, WB, IHC-Wm
  • Species reactivityZebrafish
Cat No: GTX124486
Package Price Qty
50 μlUSD 199.00
200 μlUSD 389.00
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  • Anti-Hmgb1 antibody used in IHC (Whole mount). GTX124486
  • Anti-Hmgb1 antibody used in Western Blot (WB). GTX124486
  • Anti-Hmgb1 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX124486
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  • Datasheet
  • Bio-info
  • Protocol & MSDS

  • Application Information: Hmgb1 antibody

Catalog Number GTX124486
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Publication ( 0 )
Product Name Hmgb1 antibody
See all zebrafish Hmgb1 products
ApplicationsIHC-P, WB, IHC-Wm
Application Note
Suggested dilutionReference
IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*  
Western Blot (WB)1:500-1:3000*  
IHC (Whole mount)1:100-1:500*  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Positive Controlszebrafish liver
Form SuppliedLiquid
Concentration0.43 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: Hmgb1 antibody

Full Namehigh-mobility group box 1
Synonymshigh mobility group box 1 antibody, high-mobility group box 1 antibody, high mobility group protein B1 antibody, tdsubc_1a5 antibody, fb23c02 antibody, ik:tdsubc_1a5 antibody, wu:fb23c02 antibody, xx:tdsubc_1a5 antibody, zgc:56110 antibody, zgc:77104 antibody, hmgb1 antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
Species ReactivityZebrafish
ConjugationUnconjugated

  • Storage Conditions: Hmgb1 antibody

Storage Buffer1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage InstructionStore as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20° C or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Neuroscience > Circadian rhythm
  • Zebrafish > Validated Product
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Hmgb1 antibody validated data
  • Anti-Hmgb1 antibody used in IHC (Whole mount). GTX124486
    • GTX124486 IHC-Wm Image
      Hmgb1 antibody detects Hmgb1 protein on whole mount zebrafish by immunohistochemical analysis.
      Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
      Green: Hmgb1 stained by Hmgb1 antibody (GTX124486) diluted at 1:100.
      Top
  • Anti-Hmgb1 antibody used in Western Blot (WB). GTX124486
    • GTX124486 WB Image
      Various tissue extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Hmgb1 antibody (GTX124486) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-Hmgb1 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX124486
    • GTX124486 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using hmgb1 (GTX124486) antibody at 1:300 dilution.
      Top
  • Anti-Hmgb1 antibody used in IHC (Whole mount). GTX124486
    • GTX124486 IHC-Wm Image
      Hmgb1 antibody detects Hmgb1 protein on whole mount zebrafish by immunohistochemical analysis.
      Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
      Green: Hmgb1 stained by Hmgb1 antibody (GTX124486) diluted at 1:100.
      Top
  • Anti-Hmgb1 antibody used in Western Blot (WB). GTX124486
    • GTX124486 WB Image
      Various tissue extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Hmgb1 antibody (GTX124486) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-Hmgb1 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX124486
    • GTX124486 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using hmgb1 (GTX124486) antibody at 1:300 dilution.
      Top
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Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Danio reriohmgb1a321622Q6NX86NM_199555.2NP_95584923.68
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-PBS.pdf
    MSDS-ProClin.pdf

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All products are for research use only—Not for use in diagnostic procedures.