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Homeobox C10 antibody

Anti-Homeobox C10 antibody used in IHC (Paraffin sections) (IHC-P). GTX118025
Anti-Homeobox C10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118025
Anti-Homeobox C10 antibody used in Western Blot (WB). GTX118025
Anti-Homeobox C10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118025
Anti-Homeobox C10 antibody used in IHC (Paraffin sections) (IHC-P). GTX118025
Anti-Homeobox C10 antibody used in IHC (Paraffin sections) (IHC-P). GTX118025
Anti-Homeobox C10 antibody used in Western Blot (WB). GTX118025

Cat. No. GTX118025

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Homeobox C10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

mouse muscle

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.57 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Homeobox C10. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11170594

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

homeobox C10 , HOX3I

Cellular Localization

Nucleus

Background

This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, which are located on different chromosomes and consist of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXC genes located in a cluster on chromosome 12. The protein level is controlled during cell differentiation and proliferation, which may indicate this protein has a role in origin activation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Homeobox C10 antibody used in IHC (Paraffin sections) (IHC-P). GTX118025

GTX118025 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using Homeobox C10(GTX118025) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Homeobox C10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118025

GTX118025 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Homeobox C10(GTX118025) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-Homeobox C10 antibody used in Western Blot (WB). GTX118025

GTX118025 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Homeobox C10 antibody (GTX118025) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Homeobox C10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118025

GTX118025 ICC/IF Image

Homeobox C10 antibody detects Homeobox C10 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Homeobox C10 stained by Homeobox C10 antibody (GTX118025) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar= 10 μm.

Anti-Homeobox C10 antibody used in IHC (Paraffin sections) (IHC-P). GTX118025

GTX118025 IHC-P Image

Homeobox C10 antibody detects Homeobox C10 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
Homeobox C10 stained by Homeobox C10 antibody (GTX118025) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Homeobox C10 antibody used in IHC (Paraffin sections) (IHC-P). GTX118025

GTX118025 IHC-P Image

Homeobox C10 antibody detects Homeobox C10 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Homeobox C10 stained by Homeobox C10 antibody (GTX118025) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Homeobox C10 antibody used in Western Blot (WB). GTX118025

GTX118025 WB Image

Homeobox C10 antibody detects Homeobox C10 protein by western blot analysis.
A.50 μg mouse muscle lysate/extract
10% SDS-PAGE
Homeobox C10 antibody (GTX118025) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169