Homer1 antibody

Cat. No. GTX103278

Reference 7

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Fr, IP
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all Homer1 products

View product citations for antibody GTX103278 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:10000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Fr	1:100-1:1000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS

Predict Reactivity

Bovine, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.06 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Homer1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950505

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

homer scaffold protein 1 , HOMER , HOMER1A , HOMER1B , HOMER1C , SYN47 , Ves-1

Cellular Localization

Cytoplasm , Cell junction , synapse , postsynaptic cell membrane , postsynaptic density , synapse

Background

This gene encodes a member of the homer family of dendritic proteins. Members of this family regulate group 1 metabotrophic glutamate receptor function. [provided by RefSeq]

Database

Gene ID: 9456 HOMER1
UniProt: Q86YM7 HOMER1

Research Area

DATA IMAGES

GTX103278 ICC/IF Image

Homer1 antibody detects Homer1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Homer1 protein stained by Homer1 antibody (GTX103278) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX103278 IHC-Fr Image

Homer antibody detects Homer protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Homer protein stained by Homer antibody (GTX103278) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX103278 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Homer1 antibody (GTX103278) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103278 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using HOMER1(GTX103278) antibody at 1:100 dilution.

GTX103278 IP Image

Immunoprecipitation of Homer protein from IMR32 whole cell extracts using 5 μg of Homer antibody (GTX103278).
Western blot analysis was performed using Homer antibody (GTX103278).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX103278 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Homer(GTX103278) antibody at 1:500 dilution.

GTX103278 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Homer1 antibody (GTX103278) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.