|
Catalog Number
|
GTX28693
(
0
)
|
|
Product Name
|
Human Lung (Tumor) tissue Lysate, Membrane Fraction
|
| Synonyms | Human Lung (Tumor) lysate, Membrane Fraction, Human Lung (Tumor) tissue extract, Membrane Fraction |
| Background | Human lung tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human lung tissue was frozen in liquid nitrogen immediately after excision and then stored at -70oC. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated lung tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated lung tissue membrane protein is then Western analyzed by either GAPDH or beta-actin antibody to confirm there is no signal or very weak signal. |
| Lysate Fraction | Membrane Fraction |
| Tissue | Lung |
| Cell Lines | Lung |
| Diagnosis | Tumor |
| Applications | WB |
| Application Note |
| Suggested dilution | Reference |
| Western Blot (WB) | 10 μg - 20 μg* |
|
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
|
| Form Supplied | Liquid |
| Concentration | 2
mg/ml
|
| Storage Buffer | HEPES pH7.9 with MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40. |
| Storage Instruction | Store at 2-8˚C for continuous use. For extended storage, freeze working aliquots at -20˚C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt. |
| Notes | For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |