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Catalog Number
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GTX29140
(
0
)
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Product Name
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Human Lymph (Tumor) tissue Lysate
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| Synonyms | Human Lymph (Tumor) lysate, Human Lymph (Tumor) tissue extract |
| Background | Lymphoma tumor tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% beta-mercaptoethanol. |
| Lysate Fraction | Whole Cell Extract/Total Protein |
| Cell Lines | Lymph |
| Diagnosis | Tumor |
| Applications | WB |
| Form Supplied | Liquid |
| Concentration | 2
mg/ml
|
| Storage Buffer | SDS sample buffer with 5% β-mercaptoethanol. |
| Storage Instruction | Store at 2-8˚C for continuous use. For extended storage, freeze working aliquots at -20˚C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt. |
| Notes | For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |