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Catalog Number
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GTX27457
(
0
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Product Name
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Human Raji (Human Lymphoma; B Lymphoma) (Tumor) tissue Lysate, Membrane Fraction
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| Synonyms | Human Raji (Human Lymphoma; B Lymphoma) (Tumor) lysate, Membrane Fraction, Human Raji (Human Lymphoma; B Lymphoma) (Tumor) tissue extract, Membrane Fraction |
| Background | Raji (Human lymphoma; B lymphoma) cell membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The Raji (Human lymphoma; B lymphoma) cell was frozen in liquid nitrogen immediately after excision and then stored at -70oC. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated Raji (Human lymphoma; B lymphoma) cell membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated Raji (Human lymphoma; B lymphoma) cell membrane protein is then Western analyzed by either GAPDH or beta-actin antibody to confirm there is no signal or very weak signal. |
| Lysate Fraction | Membrane Fraction |
| Cell Lines | Raji (Human Lymphoma; B Lymphoma) |
| Diagnosis | Tumor |
| Applications | WB |
| Application Note |
| Suggested dilution | Reference |
| Western Blot (WB) | 10 μg - 20 μg* |
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Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
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| Form Supplied | Liquid |
| Concentration | 2
mg/ml
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| Storage Buffer | HEPES pH7.9 with MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40. |
| Storage Instruction | Store at 2-8˚C for continuous use. For extended storage, freeze working aliquots at -20˚C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt. |
| Notes | For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |