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Catalog Number
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GTX27308
(
0
)
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Product Name
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Human Skeletal Muscle (Fetal Normal) tissue Lysate, Cytoplasmic Fraction
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| Synonyms | Human Skeletal Muscle (Fetal Normal) lysate, Cytoplasmic Fraction, Human Skeletal Muscle (Fetal Normal) tissue extract, Cytoplasmic Fraction |
| Background | human skeletal muscle tissue cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The fetal human skeletal muscle tissue was frozen in liquid nitrogen immediately after excision and then stored at -70oC. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated Skeletal Muscle tissue cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated Skeletal Muscle tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot. |
| Lysate Fraction | Cytoplasmic Fraction |
| Tissue | Muscle |
| Cell Lines | Skeletal Muscle |
| Diagnosis | Fetal Normal |
| Applications | WB |
| Application Note |
| Suggested dilution | Reference |
| Western Blot (WB) | 10 μg - 20 μg* |
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Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
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| Form Supplied | Liquid |
| Concentration | 2
mg/ml
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| Storage Buffer | HEPES pH7.9 with MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40. |
| Storage Instruction | Store at 2-8˚C for continuous use. For extended storage, freeze working aliquots at -20˚C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt. |
| Notes | For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |