Catalog Number
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GTX26390

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0
)
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Product Name
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Human Stomach: Corpus (Normal) tissue Lysate, Cytoplasmic Fraction
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Synonyms | Human Stomach: Corpus (Normal) lysate, Cytoplasmic Fraction, Human Stomach: Corpus (Normal) tissue extract, Cytoplasmic Fraction |
Background | Human stomach: corpus tissue cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The human stomach: corpus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70oC. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated stomach: corpus tissue cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated stomach: corpus tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot. |
Lysate Fraction | Cytoplasmic Fraction |
Tissue | Stomach |
Cell Lines | Stomach: Corpus |
Diagnosis | Normal |
Applications | WB |
Application Note |
| Suggested dilution | Reference |
Western Blot (WB) | 10 μg - 20 μg* |
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Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.
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Form Supplied | Liquid |
Concentration | 2
mg/ml
|
Storage Buffer | HEPES pH7.9 with MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40. |
Storage Instruction | Store at 2-8˚C for continuous use. For extended storage, freeze working aliquots at -20˚C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt. |
Notes | For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |