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Catalog Number
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GTX15376
(
0
)
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Product Name
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Human Thyroid (Normal) tissue lysate
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| ProductDescription | Human Thyroid normal tissue lysate |
| Lysate Fraction | Whole Cell Extract/Total Protein |
| Tissue | Enodcrine |
| Cell Lines | Human Thyroid Normal |
| Diagnosis | Normal |
| Applications | WB |
| Application Note | WB: The tissue lysate is ready to load on SDS-PAGE for Western blotting, 10 µg to 20 µg per lane is recommended for mini gel. Optimal dilutions/concentrations should be determined by the end user. |
| Form Supplied | Liquid |
| Purification | Whole Tissue Lysate |
| Purification Note | Human Retina normal tissue lysate was prepared by homogenization in modified RIPA buffer. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The tissue lysate was boiled for 5 min in 1 x |
| Concentration | 2
mg/ml
|
| Storage Buffer | SDS PAGE sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% beta-mercaptoethanol. |
| Storage Instruction | Upon Receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Notes | For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |