|Applications||IHC-P, IP, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||47 kDa (note)
|Positive Controls||A431 , HepG2 , JC , C2C12 , 293T , Hela , Neuro2A , GL261 , NIH-3T3 , BCL-1|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||isocitrate dehydrogenase 1 (NADP+), soluble|
|Product Description||Rabbit Polyclonal antibody to IDH1 (isocitrate dehydrogenase 1 (NADP+), soluble)|
|Background||Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. [provided by RefSeq]|
|Synonyms||IDCD antibody, IDH antibody, IDP antibody, IDPC antibody, PICD antibody, IDH1 antibody, isocitrate dehydrogenase [NADP] cytoplasmic antibody, "NADP-dependent isocitrate dehydrogenase, peroxisomal antibody", NADP(+)-specific ICDH antibody, "NADP-dependent isocitrate dehydrogenase, cytosolic antibody", oxalosuccinate decarboxylase antibody, "isocitrate dehydrogenase 1 (NADP+), soluble antibody"|
|Cellular Localization||Cytoplasm , Peroxisome |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human IDH1. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Rat, Zebrafish, Xenopus Tropicalis, Sheep, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Rat (97%), Zebrafish (85%), Xenopus Tropicalis (88%), Sheep (95%), Rhesus Monkey (100%), Bovine (95%)|