IKB alpha antibody
|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||36 kDa (note)
|Positive Controls||HepG2 , BCL-1 , C2C12 , HT-29 , NIH-3T3 , *MCF7 , *RAW264.7 , *B16F10|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha|
|Product Description||Rabbit Polyclonal antibody to IKB alpha (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha)|
|Background||NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]|
|Synonyms||IKBA antibody, MAD-3 antibody, NFKBI antibody, NFKBIA antibody, NF-kappa-B inhibitor alpha antibody, IkappaBalpha antibody, I-kappa-B-alpha antibody, nuclear factor of kappa light chain gene enhancer in B-cells antibody, ikB-alpha antibody, major histocompatibility complex enhancer-binding protein MAD3 antibody, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha antibody|
|Cellular Localization||Cytoplasm , Nucleus |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human IKB alpha. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Rat, Pig, Sheep, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Rat (91%), Pig (95%), Sheep (94%), Rhesus Monkey (99%), Bovine (94%)|