IL10 antibody [JES3-12G8] (Azide free)
|Applications||ELISA, FACS, IHC-P, WB, Functional Assay|
| ELISA||Assay-dependent dilution
| Flow cytometry/FACS||Assay-dependent dilution
|IHC (Formalin-fixed paraffin-embedded sections)||1/100*
| Functional Assay||Assay-dependent dilution
Flow cytometry/FACS : Membrane permeabilization is required for this application. Use 10μl of the suggested working dilution to label 106 cells in 100μl.Not tested in other applications.
IHC (Formalin-fixed paraffin-embedded sections) : This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
*Optimal dilutions/concentrations should be determined by the researcher.
(Please refer to the vial label for the specific concentration)|
|Purification||Protein G purified|
|Purification Note||From tissue culture supernatant|
|Full Name||interleukin 10|
|Product Description||Rat monoclonal antibody [JES3-12G8] to IL10|
|Background||The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011]|
|Synonyms||CSIF Antibody , IL10A Antibody , IL-10 Antibody , TGIF Antibody , GVHDS Antibody|
|Specificity||Clone JES3-12G8 is reported to neutralize the effects of IL-10 (Abrams et al. 1992).|
|Immunogen||Human IL-10 expressed by COS cells.|