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  • IL1RAP antibody [N1N3]

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1 .GTX104513 IL1RAP antibody [N1N3]

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IL1RAP antibody [N1N3]

Biological & Orthogonal

  • Host / ClonalityRabbit Polyclonal
  • ApplicationsIHC-P, WB
  • Species reactivityHuman, Mouse, Pig
  • Publication  ( 2 )
Cat No: GTX104513
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
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  • Anti-IL1RAP antibody [N1N3] used in Western Blot (WB). GTX104513
  • Anti-IL1RAP antibody [N1N3] used in Western Blot (WB). GTX104513
  • Anti-IL1RAP antibody [N1N3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX104513
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  • Application Information: IL1RAP antibody [N1N3]

Catalog Number GTX104513
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( 0 )
Publication ( 2 )
Product Name IL1RAP antibody [N1N3]
See all IL1RAP products
ApplicationsIHC-P, WB
Application Note
Suggested dilutionReference
IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*  
Western Blot (WB)1:500-1:3000* Y
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size65 kDa (note) IsoForm (6)
Positive ControlsHepG2 membrane extract , mouse liver
Form SuppliedLiquid
Concentration0.36 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: IL1RAP antibody [N1N3]

Full Nameinterleukin 1 receptor accessory protein
BackgroundInterleukin 1 induces synthesis of acute phase and proinflammatory proteins during infection, tissue damage, or stress, by forming a complex at the cell membrane with an interleukin 1 receptor and an accessory protein. This gene encodes an interleukin 1 receptor accessory protein. Alternative splicing of this gene results in two transcript variants encoding two different isoforms, one membrane-bound and one soluble. [provided by RefSeq]
SynonymsC3orf13 antibody, FLJ37788 antibody, IL-1RAcP antibody, IL1R3 antibody, IL1RAP antibody, IL-1R3 antibody, interleukin-1 receptor accessory protein antibody, interleukin-1 receptor accessory protein beta antibody, IL-1 receptor accessory protein antibody, interleukin-1 receptor 3 antibody, interleukin 1 receptor accessory protein antibody
Cellular LocalizationIsoform 1: Cell membrane; Single-pass type I membrane protein , Isoform 2: Secreted , Isoform 3: Secreted
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the center region of human IL1RAP. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman, Mouse, Pig
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Rat, Rhesus Monkey, Bovine
Predict Reactivity NoteRat (87%), Rhesus Monkey (99%), Bovine (89%)
ConjugationUnconjugated

  • Storage Conditions: IL1RAP antibody [N1N3]

Storage Buffer1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Disease Related > Diabetes
  • Immunology > Autoimmunity > Autoimmune diseases > Diabetes
  • Immunology > Innate immune response
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IL1RAP antibody [N1N3] validated data
  • Anti-IL1RAP antibody [N1N3] used in Western Blot (WB). GTX104513
    • GTX104513 WB Image
      Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with IL1RAP antibody [N1N3] (GTX104513) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-IL1RAP antibody [N1N3] used in Western Blot (WB). GTX104513
    • GTX104513 WB Image
      HepG2 whole cell and membrane extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IL1RAP antibody [N1N3] (GTX104513) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-IL1RAP antibody [N1N3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX104513
    • GTX104513 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded human breast cancer, using IL1RAP(GTX104513) antibody at 1:500 dilution.
      Top
  • Anti-IL1RAP antibody [N1N3] used in Western Blot (WB). GTX104513
    • GTX104513 WB Image
      Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with IL1RAP antibody [N1N3] (GTX104513) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-IL1RAP antibody [N1N3] used in Western Blot (WB). GTX104513
    • GTX104513 WB Image
      HepG2 whole cell and membrane extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IL1RAP antibody [N1N3] (GTX104513) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-IL1RAP antibody [N1N3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX104513
    • GTX104513 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded human breast cancer, using IL1RAP(GTX104513) antibody at 1:500 dilution.
      Top
Back to Top
  • Reference for IL1RAP antibody [N1N3]
Application References Application Reactivity
Zhao X et al. microRNA-4331 promotes TGEV-induced mitochondrial damage via targeting RB1, up-regulating IL1RAP, and activating p38 MAPK pathway in vitro. Mol Cell Proteomics (Epub ahead of print) (2017). WB Pig
Seo H et al. Regulatory mechanism for expression of IL1B receptors in the uterine endometrium and effects of IL1B on prostaglandin synthetic enzymes during the implantation period in pigs. Biol Reprod 87 (2):31 (2012). WB Pig
Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensIL1RAP3556Q9NPH3602626NM_001167928NP_00116140065.43
Homo sapiensIL1RAP3556Q9NPH3602626NM_001167929NP_00116140165.43
Homo sapiensIL1RAP3556Q9NPH3602626NM_001167930NP_00116140241.02
Homo sapiensIL1RAP3556Q9NPH3602626NM_001167931NP_00116140378.61
Homo sapiensIL1RAP3556Q9NPH3602626NM_002182NP_00217365.43
Homo sapiensIL1RAP3556Q9NPH3602626NM_134470NP_60827341.02
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-PBS.pdf
    MSDS-ProClin.pdf
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