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IL6 antibody

Anti-IL6 antibody used in Western Blot (WB). GTX26672
Anti-IL6 antibody used in Western Blot (WB). GTX26672

Cat. No. GTX26672

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr, Dot, ELISA, Neutralizing/Inhibition

Reactivity

 Human
Package
200 μl ($399)
See all IL6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P 1:1:100-1:200
IHC-Fr Assay dependent
Dot Assay dependent
ELISA 1:500-1:2000
Neutralizing/Inhibition 1:100
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

80 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human IL-6 produced in E.coli.

Conjugation

Unconjugated

RRID

AB_368308

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 6 , BSF-2 , BSF2 , CDF , HGF , HSF , IFN-beta-2 , IFNB2 , IL-6

Cellular Localization

Secreted

Background

Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be as important asIL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. It acts upon a variety of cells including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. In addition, IL-6appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors.

Database

Research Area

DATA IMAGES

Anti-IL6 antibody used in Western Blot (WB). GTX26672

GTX26672 WB Image

Western blot using GeneTex anti-IL6 antibody (GTX26672).~1 μg of our control protein recombinant human IL-6 was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. The blot shows detection of a band ~21 kDa in size corresponding to anti-IL6 antibody. Molecular weight markers are also shown (MW). After transfer, the membrane was blocked for 30 minutes with 1% BSA-TBST. Detection occurred using peroxidase conjugated anti-Rabbit IgG (GTX27090) secondary antibody diluted 1:40,000 in blocking buffer for 30 min at RT followed by reaction with chemiluminescent substrate.

Anti-IL6 antibody used in Western Blot (WB). GTX26672

GTX26672 WB Image

WB analysis of recombinant human IL-6 protein using GTX26672 IL6 antibody.
Lane 1 : Protein ladder
Lane 2 : 50 ng
Lane 3 : 20 ng
Lane 4 : 10 ng
Lane 5 : Mock
Dilution : 1:1000

REFERENCE

Application Reference

Dagistan Y et al. World Neurosurg 2015; 84 (6) : 1739-46 Importance of IL-6, MMP-1, IGF-1, and BAX Levels in Lumbar Herniated Disks and Posterior Longitudinal Ligament in Patients with Sciatic Pain.
Application : IHC-P
Reactivity : Human
Lemire BB et al. J Appl Physiol (1985) 2012; 113 (1) : 159-66 MAPK signaling in the quadriceps of patients with chronic obstructive pulmonary disease.
Application : IHC-Fr
Reactivity : Human
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Package List Price ($)
$ 399