PATJ antibody, N-term
|Application Note||Preliminary experiments in Human Brain and Kidney lysates consistently showed a strong band at 100kDa followed by bands at ~55kDa, 45kDa and 40kDa at 1ug/ml. Please note that currently we cannot find an explanation in the literature for the bands we observe given the predicted size of approx. 196Kda according to NP_795352.2. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Is this protein particularly susceptible to proteolysis? |
|Positive Controls||The peptide used to product this antibody is available for purchase (Catalog Number:GTX28225-PEP).|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.|
|Full Name||InaD-like (Drosophila)|
|Product Description||Goat polyclonal antibody to PATJ|
|Background||This gene encodes a protein with multiple PDZ domains. PDZ domains mediate protein-protein interactions, and proteins with multiple PDZ domains often organize multimeric complexes at the plasma membrane. This protein localizes to tight junctions and to the apical membrane of epithelial cells. A similar protein in Drosophila is a scaffolding protein which tethers several members of a multimeric signaling complex in photoreceptors. Alternative splicing results in four transcript variants encoding different isoforms. Additional transcript variants have been described but their full-length sequences have not been determined.|
|Synonyms||PALS1associated tight junction protein, PDZ domain protein, INADL, inactivation no afterpotential Dlike protein, RP4-537K17.1, PALS1-associated tight junction protein, PDZ domain protein (Drosophila inaDlike), INADL / PATJ, channelinteracting PDZ, RP4537K17.1|
|Cellular Localization||Localized in the paranodal region of myelinating Schwann cells (By similarity). Membrane-associated. Localizes to tight junctions in epithelial cells. Also found at the apical plasma membrane. |
|Specificity||This antibody is specific for PATJ.|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Dog, Pig, Bovine|