| Full Name | interferon regulatory factor 3 |
| Product Description | Mouse monoclonal antibody [4D4] to IRF3 |
| Background | Mediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE. The complex binds to the IE and PRDIII regions on the IFN-alpha and IFN-beta promoters respectively. IRF-3 does not have any transcription activation domains. |
| Cellular Localization | Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm. |
| Specificity | GTX84288 is specific for human IRF3. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Name | 4D4 |
| Target | IRF3 |
| Isotype | IgG2a |
| Immunogen | Protein expressed in 293T cell transfected with human IRF3 expression vector |
| Antigen Species | Human |
| Species Reactivity | Human |