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1 .GTX115401 IRF9 antibody

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IRF9 antibody


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, IHC-P, WB
  • Species reactivityHuman
Cat No: GTX115401
Package Price Qty
25 μlUSD 169.00
100 μlUSD 319.00
Add to cart
 
  • Anti-IRF9 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115401
  • Anti-IRF9 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX115401
  • Anti-IRF9 antibody used in Western Blot (WB). GTX115401
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  • Datasheet
  • Bio-info
  • Protocol & MSDS

  • Application Information: IRF9 antibody

Catalog Number GTX115401
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Product Name IRF9 antibody
See all IRF9 products
ApplicationsICC/IF, IHC-P, WB
Application Note
Suggested dilutionReference
Immunocytochemistry/ Immunofluorescence (ICC/IF)1:100-1:1000*  
IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*  
Western Blot (WB)1:1000-1:10000*  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size44 kDa (note) IsoForm (2)
Positive ControlsA549 , HeLa , HepG2 , HCT116
Form SuppliedLiquid
Concentration0.96 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: IRF9 antibody

Full Nameinterferon regulatory factor 9
SynonymsIRF-9 antibody, ISGF3 antibody, ISGF3G antibody, p48 antibody, IRF9 antibody, interferon regulatory factor 9 antibody, ISGF-3 gamma antibody, transcriptional regulator ISGF3 subunit gamma antibody, ISGF3 p48 subunit antibody, "interferon-stimulated transcription factor 3, gamma (48kD) antibody", IFN-alpha-responsive transcription factor subunit antibody, "interferon-stimulated transcription factor 3, gamma 48kDa antibody", interferon-stimulated gene factor 3 gamma antibody
Cellular LocalizationCytoplasm , Nucleus
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the center region of human IRF9. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
 		Mouse, Pig, Rhesus Monkey, Bovine
Predict Reactivity NoteMouse (81%), Pig (87%), Rhesus Monkey (96%), Bovine (84%)
ConjugationUnconjugated

  • Storage Conditions: IRF9 antibody

Storage Buffer0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionStore as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
IRF9 antibody validated data
  • Anti-IRF9 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115401
    • GTX115401 ICC/IF Image
      Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using IRF9(GTX115401) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
      Top
  • Anti-IRF9 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX115401
    • GTX115401 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded human breast cancer, using IRF9(GTX115401) antibody at 1:250 dilution.
      Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
      Top
  • Anti-IRF9 antibody used in Western Blot (WB). GTX115401
    • GTX115401 WB Image
      Sample (30 µg of whole cell lysate)
      A: A549
      B: HeLa
      10% SDS PAGE
      GTX115401 diluted at 1:5000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-IRF9 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115401
    • GTX115401 ICC/IF Image
      Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using IRF9(GTX115401) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
      Top
  • Anti-IRF9 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX115401
    • GTX115401 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded human breast cancer, using IRF9(GTX115401) antibody at 1:250 dilution.
      Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
      Top
  • Anti-IRF9 antibody used in Western Blot (WB). GTX115401
    • GTX115401 WB Image
      Sample (30 µg of whole cell lysate)
      A: A549
      B: HeLa
      10% SDS PAGE
      GTX115401 diluted at 1:5000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
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Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensIRF910379Q00978147574BC035716AAH3571643.71
Homo sapiensIRF910379Q00978147574NM_006084NP_00607543.71

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Mus musculusIrf916391Q61179, Q3UKQ7NM_001159417NP_00115288951.49
Mus musculusIrf916391Q61179, Q3UKQ7NM_008394NP_03242044.62
Mus musculusIrf916391Q61179, Q3UKQ7NM_001159418NP_00115289046.81
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-Thimerosal.pdf

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All products are for research use only—Not for use in diagnostic procedures.