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ISG15 antibody

KO/KD Orthogonal
Anti-ISG15 antibody used in Western Blot (WB). GTX121474
Anti-ISG15 antibody used in Western Blot (WB). GTX121474
Anti-ISG15 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121474
Anti-ISG15 antibody used in IHC (Paraffin sections) (IHC-P). GTX121474

Cat. No. GTX121474

Reference 3
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ISG15 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ISG15. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10723235

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ISG15 ubiquitin like modifier , G1P2 , IFI15 , IMD38 , IP17 , UCRP , hUCRP

Cellular Localization

Cytoplasm , Secreted

Background

ISG15 is a ubiquitin-like protein that becomes conjugated to many cellular proteins upon activation by interferon-alpha (IFNA; MIM 147660) and -beta (IFNB; MIM 147640) (Zhao et al., 2005 [PubMed 16009940]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-ISG15 antibody used in Western Blot (WB). GTX121474

GTX121474 WB Image

KO/KD

Wild-type (WT) and ISG15 knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ISG15 antibody (GTX121474) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ISG15 antibody used in Western Blot (WB). GTX121474

GTX121474 WB Image

Orthogonal

Untreated (–) and treated (+) A549 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ISG15 antibody (GTX121474) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ISG15 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121474

GTX121474 ICC/IF Image

ISG15 antibody detects ISG15 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde for 10 min.
Green: ISG15 protein stained by ISG15 antibody (GTX121474) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-ISG15 antibody used in IHC (Paraffin sections) (IHC-P). GTX121474

GTX121474 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using ISG15 antibody(GTX121474) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Zhang X et al. PLoS Pathog 2023; 19 (11) : e1011792 SARS-CoV-2 Nsp8 suppresses MDA5 antiviral immune responses by impairing TRIM4-mediated K63-linked polyubiquitination
Application : WB
Sadanari H et al. Microbiol Spectr 2022; : e0134221 The Interferon-Inducible Human PLSCR1 Protein Is a Restriction Factor of Human Cytomegalovirus.
Pons BJ et al. Cell Mol Life Sci 2021; 78 (17-18) : 6319-6335 Chronic exposure to Cytolethal Distending Toxin (CDT) promotes a cGAS-dependent type I interferon response.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

ISG15 antibody Cat. No. GTX121474

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Anti-ISG15 antibody used in Western Blot (WB). GTX121474
Date : Anonymous submitted on 04-Dec-2020
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 30 μg
Primary Antibody : 1:1000, °C, Hr
Anti-ISG15 antibody used in Western Blot (WB). GTX121474
Date : Anonymous submitted on 05-Jul-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : HEK-293 cells
Amount used : 15 μg
Blocking : 1/4-diluted ATTO EzBlock Chemi, 25°C, 0.5Hr
Primary Antibody : 1:1500, 4°C, 16Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169