|Applications||ELISA, IHC, WB|
Not tested in other applications.
|ELISA||1:2,000 - 1:10,000*
| Immunohistochemistry||Assay-dependent dilution
|Western blot||1:200 - 1:1,000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Positive Controls||Most human cell lysates can be used as a positive control without induction or stimulation|
(Please refer to the vial label for the specific concentration)|
|Purification Note||This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process, which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis. Assay by immunoelectrophoresis resulted |
|Full Name||ISG15 ubiquitin-like modifier|
|Product Description||Rabbit Polyclonal antibody to ISG15|
|Background||ISG15 a 15kDa homologue shows no amino acid sequence homology to cytokines and is synthesized as a precursor that is activated through processing by a thiol protease. ISG15 is secreted by monocytes and lymphocytes. Synthesis is induced in response to IFN alpha or IFN beta, but not IFN gamma. ISG15 expression is induced also by overexpression of some interferon regulatory factors that have been shown to play a role in the transcriptional regulation of IFN genes. ISG15 is secreted also by cell lines of monocyte (U937 cell line), T-lymphocyte, B-lymphocyte (DAUDI cells), human fibroblasts, and epithelial origins. The induction of terminal differentiation in human melanoma cells is associated, among other things, with alterations in the expression of ISG15. Intracellularly ISG15 has been shown to function as a ubiquitin homologue. It is known also as UCRP (ubiquitin cross-reactive protein). Serpin 2a (spi2a), a member of the serine protease inhibitor (serpin) protein family that is highly induced in macrophages during bacillus Calmette-Guerin infection has been shown to bind ISG15. ISG15 has been shown to modulate immune cell function. It possesses activities of cytokines and induces production of IFN gamma. It enhances proliferation and functions of natural killer and LAK cells.|
|Synonyms||IP17 Antibody , G1P2 Antibody , HUCRP Antibody , IFI15 Antibody , UCRP Antibody|
|Cellular Localization||Cytoplasmic and Secreted|
|Immunogen||This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant human ISG15 protein.|