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1 .GTX114499 ISG20 antibody

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ISG20 antibody


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, IHC-P, WB
  • Species reactivityHuman, Mouse
Cat No: GTX114499
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
Add to cart
 
  • Anti-ISG20 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX114499
  • Anti-ISG20 antibody used in Western Blot (WB). GTX114499
  • Anti-ISG20 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114499
  • Anti-ISG20 antibody used in Western Blot (WB). GTX114499
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  • Datasheet
  • Bio-info
  • Protocol & MSDS
  • Application Information: ISG20 antibody

Catalog Number GTX114499
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Product Name ISG20 antibody
See all ISG20 products
ApplicationsICC/IF, IHC-P, WB
Application Note
Suggested dilutionReference
Immunocytochemistry/ Immunofluorescence (ICC/IF)1:100-1:1000*  
IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*  
Western Blot (WB)1:500-1:3000*  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size20 kDa (note) IsoForm (5)
Positive ControlsRaji , mouse spleen
Form SuppliedLiquid
Concentration1 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: ISG20 antibody

Full Nameinterferon stimulated exonuclease gene 20kDa
BackgroundExonuclease with specificity for single-stranded RNA and, to a lesser extent for DNA. Degrades RNA at a rate that is approximately 35-fold higher than its rate for single-stranded DNA. Involved in the antiviral function of IFN against RNA viruses.
SynonymsCD25 antibody, HEM45 antibody, ISG20 antibody, estrogen-regulated transcript 45 protein antibody, promyelocytic leukemia nuclear body-associated protein ISG20 antibody, interferon-stimulated gene 20 kDa protein antibody, interferon stimulated exonuclease gene 20kDa antibody
Cellular LocalizationNucleus
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the center region of human ISG20. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman, Mouse
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Rat, Pig, Rhesus Monkey
Predict Reactivity NoteRat (82%), Pig (95%), Rhesus Monkey (97%)
ConjugationUnconjugated

  • Storage Conditions: ISG20 antibody

Storage Buffer0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ISG20 antibody validated data
  • Anti-ISG20 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX114499
    • GTX114499 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using ISG20(GTX114499) antibody at 1:500 dilution.
      Top
  • Anti-ISG20 antibody used in Western Blot (WB). GTX114499
    • GTX114499 WB Image
      Sample (30 µg of whole cell lysate)
      A: Raji
      12% SDS PAGE
      GTX114499 diluted at 1:1000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-ISG20 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114499
    • GTX114499 ICC/IF Image
      Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ISG20(GTX114499) antibody at 1:500 dilution.
      Top
  • Anti-ISG20 antibody used in Western Blot (WB). GTX114499
    • GTX114499 WB Image
      Mouse tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with ISG20 antibody (GTX114499) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
      Top
  • Anti-ISG20 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX114499
    • GTX114499 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using ISG20(GTX114499) antibody at 1:500 dilution.
      Top
  • Anti-ISG20 antibody used in Western Blot (WB). GTX114499
    • GTX114499 WB Image
      Sample (30 µg of whole cell lysate)
      A: Raji
      12% SDS PAGE
      GTX114499 diluted at 1:1000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-ISG20 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114499
    • GTX114499 ICC/IF Image
      Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ISG20(GTX114499) antibody at 1:500 dilution.
      Top
  • Anti-ISG20 antibody used in Western Blot (WB). GTX114499
    • GTX114499 WB Image
      Mouse tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with ISG20 antibody (GTX114499) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
      Top
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Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensISG203669Q96AZ6604533NP_00129016220.37
Homo sapiensISG203669Q96AZ6604533NP_00129016320.37
Homo sapiensISG203669Q96AZ6604533NP_00129016618.08
Homo sapiensISG203669Q96AZ6604533BC007922AAH0792220.37
Homo sapiensISG203669Q96AZ6604533NM_002201NP_00219220.37

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Mus musculusIsg2057444Q4FK39, Q9JL16, Q3UW00NM_001113527NP_00110699920.59
Mus musculusIsg2057444Q4FK39, Q9JL16, Q3UW00NM_020583NP_06560820.59
Mus musculusIsg2057444Q4FK39, Q9JL16, Q3UW00NP_00127815020.59
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-Thimerosal.pdf
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