APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1 - 2 μg/mL |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
3T3 (NIH) Cell Lysate(GTX27179)
Product Note
At least three isoforms of ITCH are known to exist; this antibody only recognizes the two longest isoforms. This antibody is predicted to not cross-react with other members of the Nedd4 protein family.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
ITCH antibody was raised against a 15 amino acid peptide near the amino terminus of human ITCH .The immunogen is located within amino acids 40 - 90 of ITCH.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
itchy E3 ubiquitin protein ligase , ADMFD , AIF4 , AIP4 , NAPP1
Cellular Localization
Cell membrane,Cytoplasm,Nucleus,Endosome membrane
Background
This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]
Database
Research Area
DATA IMAGES
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GTX17213 WB Image
WB analysis of 3T3 cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX17213 ITCH antibody. Working concentration : 1 μg/ml
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REFERENCE
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REVIEW
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