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Iba1 antibody

Anti-Iba1 antibody used in Western Blot (WB). GTX100042
Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100042
Anti-Iba1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX100042
Anti-Iba1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX100042
Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100042
Anti-Iba1 antibody used in Western Blot (WB). GTX100042
Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100042
Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100042
Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100042
Anti-Iba1 antibody used in Western Blot (WB). GTX100042
Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100042
Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100042
Anti-Iba1 antibody used in Flow cytometry (FACS). GTX100042
Anti-Iba1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100042
Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100042
Anti-Iba1 antibody used in Western Blot (WB). GTX100042
Anti-Iba1 antibody used in Western Blot (WB). GTX100042

Cat. No. GTX100042

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS, IHC, IHC (Free Floating)

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
FACS 1:50-1:200
IHC Assay dependent
IHC (Free Floating) Assay dependent
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

mouse liver , rat liver

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Iba1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240434

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

allograft inflammatory factor 1 , AIF-1 , IBA1 , IRT-1 , IRT1

Background

This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Iba1 antibody used in Western Blot (WB). GTX100042

GTX100042 WB Image

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (GTX100042) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100042

GTX100042 IHC-P Image

Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Iba1 stained by Iba1 antibody (GTX100042) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Iba1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX100042

GTX100042 IHC-Fr Image

Iba1 antibody detects Iba1 protein expression at microglias by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Iba1 protein stained by Iba1 antibody (GTX100042) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Iba1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX100042

GTX100042 IHC-Fr Image

Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Frozen-sectioned mouse brain.
Green: Iba1 stained by Iba1 antibody (GTX100042) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: ice-cold MeOH for 5 min

Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100042

GTX100042 IHC-P Image

Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Iba1 stained by Iba1 antibody (GTX100042) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Iba1 antibody used in Western Blot (WB). GTX100042

GTX100042 WB Image

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (GTX100042) diluted at 1:1000.

Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100042

GTX100042 IHC-P Image

Iba1 antibody detects Iba1 protein on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
Iba1 antibody (GTX100042) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100042

GTX100042 IHC-P Image

Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Iba1 stained by Iba1 antibody (GTX100042) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100042

GTX100042 IHC-P Image

Iba1 antibody detects Iba1 protein on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
Iba1 antibody (GTX100042) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Iba1 antibody used in Western Blot (WB). GTX100042

GTX100042 WB Image

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (GTX100042) diluted at 1:1000.

Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100042

GTX100042 IHC-P Image

Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
Iba1 stained by Iba1 antibody (GTX100042) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100042

GTX100042 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using Iba1(GTX100042) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Iba1 antibody detects Iba1 in the cytosol of Kupffer cell in human hepatoma by immunohistochemical analysis.

Anti-Iba1 antibody used in Flow cytometry (FACS). GTX100042

GTX100042 FACS Image

Iba1 antibody (GTX100042) detects AIF1 protein by flow cytometry analysis.
Sample: THP-1 cell.
Black: Unlabelled sample was used as a control.
Red: Iba1 antibody (GTX100042) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

Anti-Iba1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100042

GTX100042 ICC/IF Image

Iba1 antibody detects Iba1 protein at cell membrane by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Iba1 stained by Iba1 antibody (GTX100042) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100042

GTX100042 IHC-P Image

Iba1 antibody detects Iba1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
Iba1 stained by Iba1 antibody (GTX100042) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Iba1 antibody used in Western Blot (WB). GTX100042

GTX100042 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody [HL22] (GTX635363) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Iba1 antibody used in Western Blot (WB). GTX100042

GTX100042 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (GTX100042) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

REVIEW

Iba1 antibody Cat. No. GTX100042

Rating ( Average 4.9 based on 15 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 2 users reviews)
IHC (Frozen sections)(IHC-Fr) ( Average 5 based on 6 users reviews)
Immunohistochemistry(IHC) ( Average 4.7 based on 3 users reviews)
IHC (Free Floating)(IHC (Free Floating)) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 24-Jul-2023
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the 2022 in Elife. PMID: 35315776
Date : Anonymous submitted on 11-Apr-2022
Score :
Application Tested : IHC-Fr
Sample Species : Rat
Sample : Rat brain tissue section
Blocking : 2% goat serum, 20-25°C, 1Hr
Fixation : formalin (4C, 3 days) and then replace it to Sucrose
Permeabilization /Antigen retrieval : 0.1%Triton in Tris Buffered Saline
Primary Antibody : 1:250, 4°C, 12Hr
Lane Description :
Green : GTX100042
Blue : DAPI
Date : Anonymous submitted on 15-Feb-2022
Score :
Application Tested : IHC-Fr
Sample Species : Rat
Sample : Rat brain tissue section
Blocking : 10% gout serum and 0.01% Triton X-100 in PBS pH7.4, RT, 1Hr
Fixation : 4% PFA
Primary Antibody : 1:200 in blocking buffer, 4°C, 12Hr
Lane Description :
Red : GTX100042
Blue : DAPI
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal EMBO Mol Med in 2019.PMID: 31122931
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2016.PMID: 26750705
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2019.PMID: 31578352
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC (Free Floating)
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Front Immunol in 2018.PMID: 29662492
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2016.PMID: 27824125
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2016.PMID: 27824125
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Dis Model Mech in 2016. PMID: 27491075
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal J Neuroinflammation in 2018. PMID: 29753328
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal J Neuroinflammation in 2018. PMID: 29753328
Date : Anonymous submitted on 15-Dec-2020
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : brain tissue
Blocking : 1% BSA, 25°C, 0.5Hr
Fixation : 4% Paraformaldehyde
Permeabilization /Antigen retrieval : 0.4% TritonX-100, 25°C, 0.5Hr
Primary Antibody : 1:500, 4°C, 24Hr
Figure Note : Green: Iba1
Date : Anonymous submitted on 05-Jun-2018
Score :
Application Tested : IHC-Fr
Sample Species : Rat
Sample : Brain
Fixation : 4% PFA, 4°C, 48Hr
Primary Antibody : 1:100, 4°C, 16Hr
Date : Anonymous submitted on 18-Mar-2013
Score :
Application Tested : IHC
Sample Species : Ms
Sample : Mouse brain
Blocking : NGS,BSA,Trition X-100, 25°C, 2Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.1M citrate buffer(pH6.0), 95°C, 10minHr
Primary Antibody : 1:1000, 25°C, 18Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 399
$ 169