Importin 13 antibody [N1N2], N-term

Host / ClonalityRabbit Polyclonal
ApplicationsICC/IF, IHC-P, WB
Species reactivityHuman

Cat No:

GTX115274

Package	Price	Qty
25 μl	USD 119.00
100 μl	USD 319.00

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Application Information: Importin 13 antibody [N1N2], N-term

Catalog Number

GTX115274

Product Name

Importin 13 antibody [N1N2], N-term

See all Importin 13 products

Applications

ICC/IF, IHC-P, WB

Application Note

	Suggested dilution	Reference
Immunocytochemistry/ Immunofluorescence (ICC/IF)	1:100-1:1000*
IHC (Formalin-fixed paraffin-embedded sections)	1:100-1:1000*
Western Blot (WB)	1:500-1:3000*

Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.

Predicted Target Size

108 kDa (note) IsoForm

(2)

Positive Controls

human IPO13 transfected 293T

Form Supplied

Liquid

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration)

Purification

Purified by antigen-affinity chromatography.

Specifications: Importin 13 antibody [N1N2], N-term

Full Name	importin 13
Background	This gene encodes a member of the importin-beta family of nuclear transport proteins. The encoded protein mediates the import of specific cargo proteins from the cytoplasm to the nucleus and is dependent on the Ras-related nuclear protein-GTPase system. The encoded protein is also involved in nuclear export of the eukaryotic translation initiation factor 1A.
Synonyms	IMP13 antibody, KAP13 antibody, KIAA0724 antibody, LGL2 antibody, RANBP13 antibody, IPO13 antibody, ran-binding protein 13 antibody, karyopherin 13 antibody, importin-13 antibody, karyopherin-13 antibody, late gestation lung 2 antibody, Ran binding protein 13 antibody, importin 13 antibody
Cellular Localization	Cytoplasm , Nucleus
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant protein encompassing a sequence within the N-terminus region of human Importin 13. The exact sequence is proprietary.
Antigen Species	Human
Species Reactivity	Human
Predicted Cross Reactivity species Predicted cross-reactivity: Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive. Please note that we are only able to guarantee products to work in applications and species in which they have been tested.	Mouse, Rat, Chicken, Bovine
Predict Reactivity Note	Mouse (98%), Rat (98%), Chicken (82%), Bovine (98%)
Conjugation	Unconjugated

Storage Conditions: Importin 13 antibody [N1N2], N-term

Storage Buffer	1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage Instruction	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Notes	For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Importin 13 antibody [N1N2], N-term validated data

GTX115274 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using Importin 13(GTX115274) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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GTX115274 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Importin 13(GTX115274) antibody at 1:200 dilution.

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GTX115274 WB Image

Importin 13 antibody detects Importin 13 protein by western blot analysis. Non-transfected (-) and Importin 13-transfected (+, ) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Importin 13 antibody (GTX115274) diluted by 1:1000.

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GTX115274 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using Importin 13(GTX115274) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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GTX115274 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Importin 13(GTX115274) antibody at 1:200 dilution.

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GTX115274 WB Image

Importin 13 antibody detects Importin 13 protein by western blot analysis. Non-transfected (-) and Importin 13-transfected (+, ) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Importin 13 antibody (GTX115274) diluted by 1:1000.

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Target Bioinfo/ Predicted Isoform Cross Reactivity
Species	Gene name	Gene ID	SwissProt	OMIM	Isoform (mRNA)	Protein Accession#	MW
Homo sapiens	IPO13	9670	O94829	610411	BC008194	AAH08194	108.24
Homo sapiens	IPO13	9670	O94829	610411	NM_014652	NP_055467	108.22

Cross-react Bioinfo
Species	Gene name	Gene ID	SwissProt	Isoform (mRNA)	Protein Accession#	MW
Rattus norvegicus	Ipo13	116458	Q9JM04	NM_053778	NP_446230	108.21
Mus musculus	Ipo13	230673	Q8K0C1, B1ASJ5	NM_146152	NP_666264	108.25

*MSDS

MSDS-Glycerol.pdf

MSDS-PBS.pdf

MSDS-Thimerosal.pdf

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Importin 13 antibody [N1N2], N-term

Importin 13 antibody [N1N2], N-term

Application Information: Importin 13 antibody [N1N2], N-term

Specifications: Importin 13 antibody [N1N2], N-term

Storage Conditions: Importin 13 antibody [N1N2], N-term