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Influenza A virus M2 (matrix protein) antibody

Anti-Influenza A virus M2 (matrix protein) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125951
Anti-Influenza A virus M2 (matrix protein) antibody used in Western Blot (WB). GTX125951
Anti-Influenza A virus M2 (matrix protein) antibody used in Western Blot (WB). GTX125951

Cat. No. GTX125951

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, EM

Reactivity

Influenza A virus
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
EM Assay dependent
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

Positive Control

WSN-infected DF-1 cell

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.61 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus

Immunogen

Recombinant protein corresponding to Influenza A virus M2 (matrix protein) (A/Puerto Rico/8/1934(H1N1). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11170983

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Influenza A Virus Membrane 2 protein , Influenza A Virus M2

Research Area

DATA IMAGES

Anti-Influenza A virus M2 (matrix protein) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125951

GTX125951 ICC/IF Image

Influenza A Virus M2 antibody detects M2 protein at by immunofluorescent analysis.
Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min.
Green: M2 protein stained by Influenza A Virus M2 antibody (GTX125951) diluted at 1:500.
Blue: DAPI staining.
Yellow: WGA life stained at 37ºC, 30 min.

Anti-Influenza A virus M2 (matrix protein) antibody used in Western Blot (WB). GTX125951

GTX125951 WB Image

Sample (50 μg of whole cell lysate)
A: mock DF-1 cell lysates
B: WSN-infected DF-1 cell lysates
15% SDS PAGE
GTX125951 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus M2 (matrix protein) antibody used in Western Blot (WB). GTX125951

GTX125951 WB Image

Non-infected (–) and infected (+) H1299 whole cell extracts (15 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Influenza A virus M2 (matrix protein) antibody (GTX125951) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

Influenza A virus M2 (matrix protein) antibody Cat. No. GTX125951

Rating ( Average 5 based on 5 users reviews)
Application
Western Blot(WB) ( Average 5 based on 5 users reviews)
Date : Chen-I Hsu submitted on 16-Sep-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Sample : A549 cells
Amount used : 30μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:2000, 25°C, 1Hr
Secondary Antibody : GTX213110-01, 1:2000, 25°C, 1Hr
Lane Description : A549 cells were infected with Influenza A (WSN) (MOI=0.1) and harvested at 6, 24, 48 hours postinfection.
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal PLoS Pathog in 2018.PMID: 29723291
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Virol in 2017.PMID: 27795429
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Virol in 2017.PMID: 27795429
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Front Microbiol in 2017. PMID: 29312159
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169