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Influenza A virus NS1 (nonstructural protein) antibody

Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990
Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990
Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125990
Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990
Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990

Cat. No. GTX125990

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Influenza A virus
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Positive Control

WSN-infected DF-1 cell s , H1N1-infected H1299 cell

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus

Immunogen

Recombinant protein of Influenza A virus NS1 (nonstructural protein) (A/WSN/1933(H1N1). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11170327

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Influenza A Virus Non-structural protein 1 , Influenza A Virus NS1

Research Area

DATA IMAGES

Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990

GTX125990 WB Image

Influenza A viral lysate and Influenza B viral lysate (0.1 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Influenza A virus NS1 (nonstructural protein) antibody (GTX125990) diluted at 1:440. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990

GTX125990 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Influenza A virus NS1 (nonstructural protein) antibody (GTX125990) diluted at 1:440. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125990

GTX125990 ICC/IF Image

Influenza A Virus NS1 antibody detects NS1 (H1N1) protein at by immunofluorescent analysis.
Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min.
Green: NS1 (H1N1) protein stained by Influenza A Virus NS1 antibody (GTX125990) diluted at 1:500.
Blue: DAPI staining.
Yellow: WGA life stained at 37ºC, 30 min.

Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990

GTX125990 WB Image

Influenza A virus NS1 (nonstructural protein) antibody detects Influenza A virus NS1 (nonstructural protein) protein by western blot analysis. Non-infected (-) and Influenza A virus (H1N1)-infected (+) H1299 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Influenza A virus NS1 (nonstructural protein) antibody (GTX125990) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990

GTX125990 WB Image

Sample (10 μg of whole cell lysate)
A: mock DF-1 cell lysates
B: WSN-infected DF-1 cell lysates
12% SDS PAGE
GTX125990 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

Influenza A virus NS1 (nonstructural protein) antibody Cat. No. GTX125990

Rating ( Average 5 based on 9 users reviews)
Application
Western Blot(WB) ( Average 5 based on 9 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Viruses in 2019.PMID: 31500389
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal PLoS Pathog in 2018.PMID: 29723291
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2019. PMID: 31604929
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2019. PMID: 31604929
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Figure Note : The data was peer reviewed and published in the journal Front Microbiol in 2018. PMID: 30258431
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Figure Note : The data was peer reviewed and published in the journal Front Microbiol in 2018. PMID: 30258431
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Figure Note : The data was peer reviewed and published in the journal Front Microbiol in 2018. PMID: 30258431
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Figure Note : The data was peer reviewed and published in the journal Front Microbiol in 2018. PMID: 30258431
Date : Anonymous submitted on 06-Nov-2014
Score :
Application Tested : WB
Sample Species : Influenza A virus
Sample : Influenza virus-infected monkey vero cells
Amount used : 20μg
Blocking : 5% skim milk, 25°C, 1Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169