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Influenza A virus PB1 protein antibody

Anti-Influenza A virus PB1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125923
Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923
Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923

Cat. No. GTX125923

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Influenza A virus
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

Positive Control

DF1 (WSN virus treatment for 10hr)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of Influenza A virus PB1 (A/Puerto Rico/8/1934(H1N1)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2753122

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Influenza A Virus Polymerase basic protein 1 , Influenza A Virus PB1

Research Area

DATA IMAGES

Anti-Influenza A virus PB1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125923

GTX125923 ICC/IF Image

Influenza A Virus PB1 antibody detects PB1 protein at by immunofluorescent analysis.
Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min.
Green: PB1 protein stained by Influenza A Virus PB1 antibody (GTX125923) diluted at 1:500.
Blue: DAPI staining.
Yellow: WGA life stained at 37ºC, 30 min.

Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923

GTX125923 WB Image

Influenza A Virus PB1 antibody detects protein by western blot analysis.
A. 5 μg DF1 whole cell lysate/extract (untreated)
B. 5 μg DF1 whole cell lysate/extract (WSN virus treatment for 10hr)
7.5% SDS-PAGE
Influenza A Virus PB1 antibody (GTX125923) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923

GTX125923 WB Image

Non-infected (–) and infected (+) H1299 whole cell extracts (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza A virus PB1 protein antibody (GTX125923) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

Influenza A virus PB1 protein antibody Cat. No. GTX125923

Rating ( Average 5 based on 8 users reviews)
Application
Western Blot(WB) ( Average 5 based on 8 users reviews)
Date : Anonymous submitted on 19-Apr-2022
Score :
Application Tested : WB
Sample Species : Influenza A virus
Sample : MDCK cells with/without Influenza A virus (A/Puerto Rico/8/1934) infection
Amount used : 15μg
Blocking : 5% skim milk in 0.1% TBS-T, 20°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Chen-I Hsu submitted on 16-Sep-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Sample : A549 cells
Amount used : 30μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Secondary Antibody : GTX213110-01, 1:2000, 25°C, 1Hr
Lane Description : A549 cells were infected with Influenza A (WSN) (MOI=0.1) and harvested at 6, 24, 48 hours postinfection.
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Virol in 2018.PMID: 29118119
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Virol in 2018.PMID: 30209172
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Virol in 2018.PMID: 29118119
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Virol in 2018.PMID: 30209172
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017. PMID: 28842649
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Figure Note : The data was peer reviewed and published in the journal J Virol in 2019. PMID: 31068418
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169