|IHC (Frozen sections)||1/50-1/100*
|IHC (Formalin-fixed paraffin-embedded sections)||1/50-1/100*
| Western blot||Assay-dependent dilution
IHC (Formalin-fixed paraffin-embedded sections) : This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.Not tested in other applications.
Western blot : This antibody recognizes the alpha subunit of the 32 kDa human inhibin. In western blotting of bovine follicular fluid, bands at 20, 44 and 57 kDa were reported under reducing conditions using clone R1 (Groome et al, 1991).
*Optimal dilutions/concentrations should be determined by the researcher.