Integrin alpha 2 antibody [16B4]
|Applications||ELISA, FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB, Functional Assay|
| Immunocytochemistry/ Immunofluorescence||Assay-dependent dilution
|IHC (Frozen sections)||30-40μg/ml*
| IHC (Formalin-fixed paraffin-embedded sections)||Assay-dependent dilution
| Western blot||Assay-dependent dilution
| Functional Assay||Assay-dependent dilution
Flow cytometry/FACS : Use 10μl of the suggested working dilution to label 106 cells in 100μl.Not tested in other applications.
IHC (Formalin-fixed paraffin-embedded sections) : This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Tris/EDTA pH9.0 is recommended for this purpose.
Functional Assay : This product contains sodium azide. The removal by dialysis is recommended prior to use in functional assays.
*Optimal dilutions/concentrations should be determined by the researcher.
(Please refer to the vial label for the specific concentration)|
|Purification||Protein G purified|
|Purification Note||From tissue culture supernatant|
|Full Name||integrin subunit alpha 2|
|Product Description||Mouse monoclonal antibody [16B4] to Integrin alpha 2|
|Background||This gene encodes the alpha subunit of a transmembrane receptor for collagens and related proteins. The encoded protein forms a heterodimer with a beta subunit and mediates the adhesion of platelets and other cell types to the extracellular matrix. Loss of the encoded protein is associated with bleeding disorder platelet-type 9. Antibodies against this protein are found in several immune disorders, including neonatal alloimmune thrombocytopenia. This gene is located adjacent to a related alpha subunit gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]|
|Synonyms||HPA-5 Antibody , CD49B Antibody , VLAA2 Antibody , VLA-2 Antibody , GPIA Antibody , ITGA2 Antibody , BR Antibody|
|Specificity||This antibody recognizes human Integrin alpha 2.|
|Immunogen||Purified human beta 1 preparation from HT1080 fibrosarcoma cell extract.|