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Catalog Number
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GTX48181-PRO
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0
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Product Name
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Integrin alpha 4 Recombinant Protein
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| Full Name | integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor) |
| Synonyms | CD49d, ITGA4 |
| Background | The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha 4 chain associates with either beta 1 or beta 7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin alpha 4 beta 1 and alpha 4 beta 7 is located on the alpha 4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha 4 beta 7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha 4 beta 7, the gut mucosal homing receptor for peripheral T cells. This recombinant protein fragment includes the ligand and monoclonal antibody binding domains; this binding has been tested and verified in ELISA assays. This recombinant protein is recognized PSI antibodies Integrin 4 alpha (IN1) (PSI 4827) and Integrin 4 alpha (IN2) (PSI 4837), but not Integrin 4 alpha (CT) (PSI 4783). |
| Species | Human |
| Region/Sequence | a.a. 131 - 275 |
| Expression System | E. coli |
| Applications | ELISA, WB |
| Application Note |
| Suggested dilution | Reference |
| ELISA | Assay-dependent dilution |
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| Western Blot (WB) | Assay-dependent dilution |
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Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
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| Conjugation | Unconjugated |
| Form Supplied | Liquid |
| Purity | ~95% |
| Concentration | Batch dependent
(Please refer to the vial label for the specific concentration) |
| Storage Buffer | 50mM Tris pH7.5 with 150mM NaCl, 5 mM MgCl2 |
| Storage Instruction | The lyophilized recombinant protein is stable for at least 2 years from date of receipt at -20˚C. After reconstitution, it is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. Avoid multiple freeze-thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. |
| Notes | For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |
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