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1 .GTX48181-PRO Integrin alpha 4 Recombinant Protein

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Integrin alpha 4 Recombinant Protein


  • ApplicationsELISA, WB
Cat No: GTX48181-PRO
Package Price Qty
50 μgUSD 299.00
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  • Application Information: Integrin alpha 4 Recombinant Protein

Catalog Number GTX48181-PRO
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Product Name Integrin alpha 4 Recombinant Protein
See all Integrin alpha 4 products
Full Nameintegrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
SynonymsCD49d, ITGA4
BackgroundThe integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha 4 chain associates with either beta 1 or beta 7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin alpha 4 beta 1 and alpha 4 beta 7 is located on the alpha 4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha 4 beta 7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha 4 beta 7, the gut mucosal homing receptor for peripheral T cells. This recombinant protein fragment includes the ligand and monoclonal antibody binding domains; this binding has been tested and verified in ELISA assays. This recombinant protein is recognized PSI antibodies Integrin 4 alpha (IN1) (PSI 4827) and Integrin 4 alpha (IN2) (PSI 4837), but not Integrin 4 alpha (CT) (PSI 4783).
SpeciesHuman
Region/Sequencea.a. 131 - 275
Expression SystemE. coli
ApplicationsELISA, WB
Application Note
Suggested dilutionReference
ELISAAssay-dependent dilution  
Western Blot (WB)Assay-dependent dilution  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
ConjugationUnconjugated
Form SuppliedLiquid
Purity~95%
ConcentrationBatch dependent (Please refer to the vial label for the specific concentration)
Storage Buffer50mM Tris pH7.5 with 150mM NaCl, 5 mM MgCl2
Storage InstructionThe lyophilized recombinant protein is stable for at least 2 years from date of receipt at -20˚C. After reconstitution, it is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. Avoid multiple freeze-thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Cell Biology > Cell adhesion > Cell-cell adhesion > Heterophilic
  • Cell Biology > Cell adhesion > Cell-cell adhesion > Leukocyte
  • Cell Biology > Cell adhesion > Integrins
  • Immunology > Adaptive Immunity > B cell > Development
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Integrin alpha 4 Recombinant Protein validated data
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