Integrin beta 4 antibody [450-9D] (Azide free)
|Applications||FACS, ICC/IF, IHC-Fr, IP, WB|
| Immunocytochemistry/ Immunofluorescence||Assay-dependent dilution
|IHC (Frozen sections)||1/500-1/1,000*
| Immunoprecipitation||Assay-dependent dilution
| Western blot||Assay-dependent dilution
Flow cytometry/FACS : Use 10μl of the suggested working dilution to label 106 cells in 100μl.Not tested in other applications.
IHC (Frozen sections) : The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Recommend the use of acetone fixation for frozen sections.
*Optimal dilutions/concentrations should be determined by the researcher.
(Please refer to the vial label for the specific concentration)|
|Purification||Protein G purified|
|Purification Note||From tissue culture supernatant|
|Full Name||integrin subunit beta 4|
|Product Description||Mouse monoclonal antibody [450-9D] to Integrin beta 4 |
|Background||Integrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Mutations in this gene are associated with epidermolysis bullosa with pyloric atresia. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]|
|Synonyms||ITGB4 Antibody , CD104 Antibody|
|Specificity||This antibody recognizes an extracellular epitope on Integrin beta 4.|
|Immunogen||Purified alpha6 beta4 integrin from A431 cells.|
|Species Reactivity||Human, Ferret, Mustelid|