|Applications||IHC, IHC-P, WB|
Not tested in other applications.
| Immunohistochemistry||Assay-dependent dilution
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||33 kDa (note)
|Positive Controls||A431 , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||junctional adhesion molecule 2|
|Product Description||Rabbit Polyclonal antibody to JAM-B (junctional adhesion molecule 2)|
|Background||Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. It acts as an adhesive ligand for interacting with a variety of immune cell types and may play a role in lymphocyte homing to secondary lymphoid organs. [provided by RefSeq]|
|Synonyms||C21orf43 antibody, CD322 antibody, JAM-B antibody, JAMB antibody, PRO245 antibody, VE-JAM antibody, VEJAM antibody, JAM2 antibody, JAM-IT/VE-JAM antibody, JAM-2 antibody, junctional adhesion molecule B antibody, vascular endothelial junction-associated molecule antibody, junctional adhesion molecule 2 antibody|
|Cellular Localization||Cell junction , tight junction (By similarity) , Cell membrane; Single-pass type I membrane protein (By similarity) |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human JAM-B. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Predict Reactivity Note||Rat (81%), Bovine (90%)|