Jurkat (human [E6-1] T lymphocyte; acute T cell leukemia) cell line slides
|Product Description||Jurkat cell line slides [E6-1] (human: T lymphocyte; acute T cell leukemia)|
|Background||Jurkat, Clone E6-1 (T lymphocyte; acute T cell leukemia)|
Human Jurkat, Clone E6-1 cells were cultured in RPMI 1640 medium with 2 mM L-glutamine and harvested at the log phase of growth. In order to keep the antigens in their native forms, cells were fixed in 4% paraformaldehyde. The Jurkat cells were arrayed on a 12-well (5 mm) adhesive coated slide, with each wellis surface specifically treated to enhance cellular attachment and to minimize background staining. Each well was loaded with approximately 7.5 x 103 Jurkat cells. A follow-up heat dehydration process was done to ensure the attachment of cells and the stability of cellular proteins.
|Application Note||Jurkat cell slide is provided for immunocytochemical analysis of protein and protein-protein interaction. Before applying immunocytochemistry, 0.1% Triton X-100 pretreatment for 1 minute at room temperature is highly suggested. Optimal conditions should be determined by the end user.|
|Storage instruction||Jurkat cell slide can be stored for up to 6 months at 4°C under desiccate conditions.|
|Notes||For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.|